Conversion of bovine pancreatic DNase I to a repair endonuclease with a high selectivity for abasic sites
Bovine pancreatic deoxyribonuclease I (DNase I) is a nuclease of relatively low specificity which interacts with DNA in the minor groove. No contacts are made between the protein and the major groove of the nucleic acid. DNase I is structurally homologous to exonuclease III, a DNA‐repair enzyme with...
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Published in | The EMBO journal Vol. 17; no. 23; pp. 7128 - 7138 |
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Main Authors | , , , |
Format | Journal Article |
Language | English |
Published |
Chichester, UK
John Wiley & Sons, Ltd
01.12.1998
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Subjects | |
Online Access | Get full text |
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Summary: | Bovine pancreatic deoxyribonuclease I (DNase I) is a nuclease of relatively low specificity which interacts with DNA in the minor groove. No contacts are made between the protein and the major groove of the nucleic acid. DNase I is structurally homologous to exonuclease III, a DNA‐repair enzyme with multiple activities. One of the main differences between the two enzymes is the presence of an additional α‐helix in exonuclease III, in a position suggestive of interaction with the major groove of DNA. Recombinant DNA techniques have been used to add 14 amino acids, comprising the 10 amino acids of the exonuclease III α‐helix flanked by a glycine rich region, to DNase I. The polypeptide has been inserted after serine 174, an amino acid on the surface of DNase I corresponding to the location of the extra α‐helix in exonuclease III. The recombinant protein, DNase–exohelix, has been purified and its catalytic activities towards DNA investigated. The recombinant protein demonstrated a high selectivity for endonucleolytic cleavage at abasic sites in DNA, a property of exonuclease III but not native DNase I. Thus the insertion of 14 amino acids at Ser174, converts DNase I to an exonuclease III‐like enzyme with DNA‐repair properties. |
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Bibliography: | ark:/67375/WNG-9QV5G9P0-V istex:5B2AA2B6C3B9AD138E3749AB98B69DCCA801A09A ArticleID:EMBJ7591401 ObjectType-Article-2 SourceType-Scholarly Journals-1 ObjectType-Feature-1 content type line 23 |
ISSN: | 0261-4189 1460-2075 |
DOI: | 10.1093/emboj/17.23.7128 |