Effects of nitrogen form on growth, CO2 assimilation, chlorophyll fluorescence, and photosynthetic electron allocation in cucumber and rice plants

• Published in: Journal of Zhejiang University. B. Science Vol. 12; no. 2; pp. 126 - 134
• Main Authors: Zhou, Yan-hong, Zhang, Yi-li, Wang, Xue-min, Cui, Jin-xia, Xia, Xiao-jian, Shi, Kai, Yu, Jing-quan
• Format: Journal Article
• Language: English
• Published: Heidelberg SP Zhejiang University Press 01.02.2011; Springer Nature B.V; Zhejiang University Press
• Subjects: Allocations; Biomedical and Life Sciences; Biomedicine; Biotechnology; Carbon dioxide; Carbon Dioxide - metabolism; Chlorophyll - metabolism; Chlorophyll A; Chlorophylls; Cucumbers; Cucumis sativus - growth & development; Cucumis sativus - metabolism; Electron density; Electron Transport; Fluorescence; NADP - metabolism; Nitrate Reductase - metabolism; Nitrates - metabolism; Nitrogen - metabolism; Oryza - growth & development; Oryza - metabolism; Photosynthesis; Photosystem II Protein Complex - metabolism; Quaternary Ammonium Compounds - metabolism; Reduction; Rice; Nitrogen form; Photosynthetic electron allocation; Nitrate reductase; S143.1; Alternative electron flux
• ISSN: 1673-1581; 1862-1783
• DOI: 10.1631/jzus.B1000059

Cucumber and rice plants with varying ammonium （NH4＋） sensitivities were used to examine the effects of different nitrogen （N） sources on gas exchange, chlorophyll （Chl） fluorescence quenching, and photosynthetic electron allocation. Compared to nitrate （NO3-）-grown plants, cucumber plants grown under NH4＋-nutrition showed decreased plant growth, net photosynthetic rate, stomatal conductance, intercellular carbon dioxide （CO2） level, transpiration rate, maximum photochemical efficiency of photosystem II, and O2-independent alternative electron flux, and increased O2-dependent alternative electron flux. However, the N source had little effect on gas exchange, Chl a fluorescence parameters, and photosynthetic electron allocation in rice plants, except that NH4＋-grown plants had a higher O2-independent alternative electron flux than NO3--grown plants. NO3- reduction activity was rarely detected in leaves of NH4＊-grown cucumber plants, but was high in NH4＋-grown rice plants. These results demonstrate that significant amounts of photosynthetic electron transport were coupled to NO3- assimilation, an effect more significant in NO3-- grown plants than in NH4＋-grown plants. Meanwhile, NH4＊-tolerant plants exhibited a higher demand for the reduced form of nicotinamide adenine dinucleotide phosphate （NADPH） for NO3- reduction, regardless of the N form supplied, while NH4＋-sensitive plants had a high water-water cycle activity when NH4＋ was supplied as the sole N source.