Multiple infection of porcine Torque teno virus in a single pig and characterization of the full-length genomic sequences of four U.S. prototype PTTV strains: Implication for genotyping of PTTV

• Published in: Virology (New York, N.Y.) Vol. 396; no. 2; pp. 289 - 297
• Main Authors: Huang, Y.W, Ni, Y.Y, Dryman, B.A, Meng, X.J
• Format: Journal Article
• Language: English
• Published: United States Elsevier Inc 20.01.2010
• Subjects: Amino Acid Sequence; Anellovirus; Animals; Conserved Sequence - genetics; DNA Virus Infections - genetics; DNA Virus Infections - veterinary; Genome, Viral - genetics; Genomic sequences; Genotype; Genotyping; Infectious Disease; Male; Multiple infection; Open Reading Frames - genetics; Phylogeny; Pig; Porcine TTV (PTTV); Sequence Alignment; Species Specificity; Swine - virology; Swine Diseases - virology; Torque teno virus (TTV); Torque teno virus - genetics; United States; Viral Proteins - genetics; United States; Torque teno virus (TTV); PCV; PTTV; Torque teno mini virus; Genomic sequences; Torque teno virus; untranslated region; chicken anemia virus; Multiple infection; TTMV; porcine circovirus; Pig; porcine Torque teno virus; UTR; Genotyping; Torque teno midi virus; TTV; TTMDV; CAV; Porcine TTV (PTTV); Anellovirus
• ISSN: 0042-6822; 1096-0341
• DOI: 10.1016/j.virol.2009.10.031

Abstract Porcine Torque teno virus (PTTV) was recently shown to partially contribute to the experimental induction of porcine dermatitis and nephropathy syndrome and postweaning multisystemic wasting syndrome in pigs in the United States. We report here the identification of four distinct full-length genomic sequences of PTTV strains from a single pig in Virginia. Detailed analyses of the genomic organization, the degree of variability and the characteristics of conserved nucleotide and amino acid motifs of PTTV were conducted. The results showed that these four prototype U.S. strains of PTTV identified from the same pig represent distinct genotypes or subtypes and a revised classification system for PPTV is subsequently proposed. This is the first study documenting multiple PTTV infections with distinct genotypes or subtypes in a single pig. The identification of novel PTTV strains from pigs in the United States also pave the way for future disease characterization and genotyping of PTTV.