Activation of endogenous neurogenesis and angiogenesis by basic fibroblast growth factor-chitosan gel in an adult rat model of ischemic stroke

• Published in: Neural regeneration research Vol. 19; no. 2; pp. 409 - 415
• Main Authors: Duan, Hongmei, Li, Shulun, Hao, Peng, Hao, Fei, Zhao, Wen, Gao, Yudan, Qiao, Hui, Gu, Yiming, Lv, Yang, Bao, Xinjie, Chiu, Kin, So, Kwok-Fai, Yang, Zhaoyang, Li, Xiaoguang
• Format: Journal Article
• Language: English
• Published: India Medknow Publications & Media Pvt. Ltd 01.02.2024; Department of Biomedical Engineering,School of Biological Science and Medical Engineering,Beihang University,Beijing,China; Department of Ophthalmology and State Key Laboratory of Brain and Cognitive Sciences,The University of Hong Kong,Hong Kong Special Administration Region,China; Bioland Laboratory(Guangzhou Regenerative Medicine and Health Guangdong Laboratory),Guangzhou,Guangdong Province,China; Center for Brain Science and Brain-Inspired Intelligence,Guangdong-Hong Kong-Macao Greater Bay Area,Guangzhou,Guangdong Province,China; Department of Neurobiology,School of Basic Medical Sciences,Capital Medical University,Beijing,China%Beijing Key Laboratory for Biomaterials and Neural Regeneration,School of Engineering Medicine,Beihang University,Beijing,China%Department of Epidemiology and Statistics,School of Public Health and Management,Ningxia Medical University,Yinchuan,Ningxia Hui Autonomous Region,China%Department of Physical Education,Capital University of Economics and Businessm,Beijing,China%Department of Neurosurgery,Peking Union Medical College Hospital,Chinese Academy of Medical Sciences and Peking Union Medical College,Beijing,China%Department of Psychology,State Key Lab of Brain and Cognitive Sciences,The University of Hong Kong,Hong Kong Special Administration Region,China%Guangdong-Hongkong-Macau Institute of CNS Regeneration,Ministry of Education CNS Regeneration Collaborative Joint Laboratory,Jinan University,Guangzhou,Guangdong Province,China; Co-innovation Center of Neuroregeneration,Nantong University,Nantong,Jiangsu Province,China%Department of Neurobiology,School of Basic Medical Sciences,Capital Medical University,Beijing,China; Wolters Kluwer - Medknow; Wolters Kluwer Medknow Publications
• Subjects: adult endogenous neurogenesis; angiogenesis; basic fibroblast growth factor-chitosan gel; chitosan; functional recovery; ischemic stroke; neural stem cell; newborn neuron; Angiogenesis; Fibroblasts; Growth factors; Ischemia; Neurogenesis; adult endogenous neurogenesis; chitosan; neural stem cell; angiogenesis; newborn neuron; functional recovery; basic fibroblast growth factor-chitosan gel; ischemic stroke
• ISSN: 1673-5374; 1876-7958
• DOI: 10.4103/1673-5374.375344

JOURNAL/nrgr/04.03/01300535-202402000-00035/inline-graphic1/v/2023-07-19T141749Z/r/image-tiff Attempts have been made to use cell transplantation and biomaterials to promote cell proliferation, differentiation, migration, and survival, as well as angiogenesis, in the context of brain injury. However, whether bioactive materials can repair the damage caused by ischemic stroke by activating endogenous neurogenesis and angiogenesis is still unknown. In this study, we applied chitosan gel loaded with basic fibroblast growth factor to the stroke cavity 7 days after ischemic stroke in rats. The gel slowly released basic fibroblast growth factor, which improved the local microenvironment, activated endogenous neural stem/progenitor cells, and recruited these cells to migrate toward the penumbra and stroke cavity and subsequently differentiate into neurons, while enhancing angiogenesis in the penumbra and stroke cavity and ultimately leading to partial functional recovery. This study revealed the mechanism by which bioactive materials repair ischemic strokes, thus providing a new strategy for the clinical application of bioactive materials in the treatment of ischemic stroke.