FRET-based modified graphene quantum dots for direct trypsin quantification in urine

• Published in: Analytica chimica acta Vol. 917; pp. 64 - 70
• Main Authors: Poon, Chung-Yan, Li, Qinghua, Zhang, Jiali, Li, Zhongping, Dong, Chuan, Lee, Albert Wai-Ming, Chan, Wing-Hong, Li, Hung-Wing
• Format: Journal Article
• Language: English
• Published: Netherlands Elsevier B.V 21.04.2016
• Subjects: Biosensing Techniques; BSA; Coumarin; Derivatives; Emission; Fluorescence; Fluorescence Resonance Energy Transfer - methods; FRET; Fretting; GQD; Graphene; Graphite - chemistry; Humans; Limit of Detection; Microscopy, Electron, Transmission; Quantum Dots; Trypsin; Trypsin - urine; Urine; BSA; Trypsin; FRET; GQD; Coumarin
• ISSN: 0003-2670; 1873-4324
• DOI: 10.1016/j.aca.2016.02.032

A versatile nanoprobe was developed for trypsin quantification with fluorescence resonance energy transfer (FRET). Here, fluorescence graphene quantum dot is utilized as a donor while a well-designed coumarin derivative, CMR2, as an acceptor. Moreover, bovine serum albumin (BSA), as a protein model, is not only served as a linker for the FRET pair, but also a fluorescence enhancer of the quantum dots and CMR2. In the presence of trypsin, the FRET system would be destroyed when the BSA is digested by trypsin. Thus, the emission peak of the donor is regenerated and the ratio of emission peak of donor/emission peak of acceptor increased. By the ratiometric measurement of these two emission peaks, trypsin content could be determined. The detection limit of trypsin was found to be 0.7 μg/mL, which is 0.008-fold of the average trypsin level in acute pancreatitis patient's urine suggesting a high potential for fast and low cost clinical screening. [Display omitted] •A FRET-based biosensor was developed for direct quantification of trypsin.•Fast and sensitive screening of pancreatic disease was facilitated.•The direct quantification of trypsin in urine samples was demonstrated.