Cell cycle progression and de novo centriole assembly after centrosomal removal in untransformed human cells

How centrosome removal or perturbations of centrosomal proteins leads to G1 arrest in untransformed mammalian cells has been a mystery. We use microsurgery and laser ablation to remove the centrosome from two types of normal human cells. First, we find that the cells assemble centrioles de novo afte...

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Bibliographic Details
Published inThe Journal of cell biology Vol. 176; no. 2; pp. 173 - 182
Main Authors Uetake, Yumi, Lončarek, Jadranka, Nordberg, Joshua J, English, Christopher N, La Terra, Sabrina, Khodjakov, Alexey, Sluder, Greenfield
Format Journal Article
LanguageEnglish
Published United States The Rockefeller University Press 15.01.2007
Rockefeller University Press
Subjects
Bromodeoxyuridine - metabolism
Calcium-Binding Proteins - analysis
Cell cycle
Cell Cycle - drug effects
Cell Cycle - physiology
Cell Cycle - radiation effects
Cells
Cells, Cultured
Centrioles
Centrioles - chemistry
Centrioles - physiology
Centrioles - ultrastructure
Centrosome - physiology
Centrosomes
Chromosomal Proteins, Non-Histone - analysis
Daughter cells
Enzyme Inhibitors - pharmacology
Epithelial cells
Epithelial Cells - cytology
Epithelial Cells - metabolism
Epithelial Cells - ultrastructure
G1 Phase - physiology
HeLa cells
Humans
Imidazoles - pharmacology
Interphase
Lasers
Light
Microscopy, Electron
Microsurgery
Mitosis
p38 Mitogen-Activated Protein Kinases - antagonists & inhibitors
p38 Mitogen-Activated Protein Kinases - metabolism
Proteins
Pyridines - pharmacology
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Summary:How centrosome removal or perturbations of centrosomal proteins leads to G1 arrest in untransformed mammalian cells has been a mystery. We use microsurgery and laser ablation to remove the centrosome from two types of normal human cells. First, we find that the cells assemble centrioles de novo after centrosome removal; thus, this phenomenon is not restricted to transformed cells. Second, normal cells can progress through G1 in its entirety without centrioles. Therefore, the centrosome is not a necessary, integral part of the mechanisms that drive the cell cycle through G1 into S phase. Third, we provide evidence that centrosome loss is, functionally, a stress that can act additively with other stresses to arrest cells in G1 in a p38-dependent fashion.
Bibliography:ObjectType-Article-1
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Correspondence to Greenfield Sluder: Greenfield.sluder@umassmed.edu
Abbreviation used in this paper: HMEC, human mammary epithelial cell.
ISSN:0021-9525
1540-8140
DOI:10.1083/jcb.200607073