A conserved 5‘ to 3‘ exonuclease activity in the yeast and human nucleotide excision repair proteins RAD2 and XPG

Saccharomyces cerevisiae RAD2 protein and its human homolog xeroderma pigmentosum group G (XPG) protein function in the incision step of nucleotide excision repair of DNA damaged by ultraviolet light. Both RAD2 and XPG proteins have been shown previously to possess an endonuclease activity. Using DN...

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Bibliographic Details
Published inThe Journal of biological chemistry Vol. 269; no. 50; pp. 31342 - 31345
Main Authors Habraken, Y, Sung, P, Prakash, L, Prakash, S
Format Journal Article
LanguageEnglish
Published United States Elsevier Inc 16.12.1994
American Society for Biochemistry and Molecular Biology
Subjects
ACTIVIDAD ENZIMATICA
ACTIVITE ENZYMATIQUE
ADN
Base Sequence
BIOLOGICAL DIFFERENCES
DIFERENCIAS BIOLOGICAS
DIFFERENCE BIOLOGIQUE
DNA
DNA MODIFICATION
DNA REPAIR
DNA-Binding Proteins - metabolism
DOUBLE STRANDED DNA
Endodeoxyribonucleases
Endonucleases
ENZYMIC ACTIVITY
Exonucleases - metabolism
Fungal Proteins - metabolism
GENERO HUMANO
GENRE HUMAIN
Humans
MANKIND
Molecular Sequence Data
Nuclear Proteins
NUCLEASAS
NUCLEASE
NUCLEASES
Oligodeoxyribonucleotides - chemistry
RAD2 PROTEIN
RADIACION ULTRAVIOLETA
RAYONNEMENT ULTRAVIOLET
Recombinant Proteins
SACCHAROMYCES CEREVISIAE
Saccharomyces cerevisiae - enzymology
Saccharomyces cerevisiae Proteins
SINGLE STRANDED DNA
SPECIES DIFFERENCES
Transcription Factors
ULTRAVIOLET RADIATION
Xeroderma Pigmentosum - enzymology
XPG PROTEIN
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Summary:Saccharomyces cerevisiae RAD2 protein and its human homolog xeroderma pigmentosum group G (XPG) protein function in the incision step of nucleotide excision repair of DNA damaged by ultraviolet light. Both RAD2 and XPG proteins have been shown previously to possess an endonuclease activity. Using DNA substrates labeled at either the 5' end or 3' end, we now demonstrate that RAD2 protein also digests both single-stranded and double-stranded DNAs exonucleolytically with a 5' to 3' directionality. A 5' to 3' exonuclease activity is also present in the XPG protein, indicating evolutionary conservation of this activity. The possible role of RAD2 and XPG 5' to 3' exonuclease activity in nucleotide excision repair is discussed.
Bibliography:1997053951
F30
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ISSN:0021-9258
1083-351X
DOI:10.1016/S0021-9258(18)31699-5