High-Cell-Density Cyclic Fed-Batch Fermentation of a Poly(3-Hydroxybutyrate)-Accumulating Thermophile, Chelatococcus sp. Strain MW10

• Published in: Applied and Environmental Microbiology Vol. 76; no. 23; pp. 7890 - 7895
• Main Authors: Ibrahim, Mohammad H.A, Steinbüchel, Alexander
• Format: Journal Article
• Language: English
• Published: Washington, DC American Society for Microbiology 01.12.2010; American Society for Microbiology (ASM)
• Subjects: Bacteria; Batch culture; batch fermentation; Beijerinckiaceae - growth & development; Beijerinckiaceae - metabolism; Biological and medical sciences; Bioreactors - microbiology; Biotechnology; Cell cycle; Cell growth; Cells; Chelatococcus; Culture Media - chemistry; Fermentation; Fundamental and applied biological sciences. Psychology; Hydroxybutyrates - metabolism; Methods. Procedures. Technologies; Microbial engineering. Fermentation and microbial culture technology; Microbiology; Polyesters - metabolism; polyhydroxybutyrate; thermophilic bacteria; Butyrate(hydroxy)polymer; Thermophily; Fermentation; High density; Semicontinuous
• ISSN: 0099-2240; 1098-5336; 1098-6596
• DOI: 10.1128/AEM.01488-10

Different fermentation strategies were employed for the cultivation of a new poly(3-hydroxybutyrate)-accumulating thermophilic bacterium, Chelatococcus sp. strain MW10, with the aim of achieving high-cell-density (HCD) growth and high poly(3-hydroxybutyrate) [poly(3HB)] productivity. Enhanced cultivation was achieved by a cyclic fed-batch fermentation (CFBF) technique (42-liter scale). Maximal poly(3HB) productivity was obtained during the second cycle [16.8 ± 4.2 g poly(3HB)/liter]. At the end of CFBF (265 h), an HCD of up to 115.0 ± 4.3 g cell dry weight/liter was achieved.