Rapid resonance Raman microspectroscopy to probe carbon dioxide fixation by single cells in microbial communities

• Published in: The ISME Journal Vol. 6; no. 4; pp. 875 - 885
• Main Authors: Li, Mengqiu, Canniffe, Daniel P, Jackson, Philip J, Davison, Paul A, FitzGerald, Simon, Dickman, Mark J, Burgess, J Grant, Hunter, C Neil, Huang, Wei E
• Format: Journal Article
• Language: English
• Published: London Nature Publishing Group UK 01.04.2012; Nature Publishing Group
• Subjects: Aquatic ecosystems; Aquatic microorganisms; Biomedical and Life Sciences; Carbon dioxide; Carbon Dioxide - metabolism; Carbon dioxide fixation; Carotenoids; Chemical analysis; Ecological function; Ecology; Ecophysiology; Evolutionary Biology; imaging; Life Sciences; Marine ecosystems; Marine environment; Mass spectrometry; Mass spectroscopy; Microalgae; Microbial activity; Microbial Ecology; Microbial Genetics and Genomics; Microbiology; Microorganisms; Mixed culture; Natural environment; Original; original-article; Photosynthesis; Pigments; Probes; Resonance; Seawater; Seawater - microbiology; Single-Cell Analysis - methods; Spectrum Analysis, Raman - methods; Synechococcus - metabolism; Synechocystis - metabolism; Water analysis; single cell; carotenoids; photosynthesis; Raman imaging; resonance Raman; carbon dioxide fixation

Photosynthetic microorganisms play crucial roles in aquatic ecosystems and are the major primary producers in global marine ecosystems. The discovery of new bacteria and microalgae that play key roles in CO 2 fixation is hampered by the lack of methods to identify hitherto-unculturable microorganism...