Spatial versus temporal laser speckle contrast analyses in the presence of static optical scatterers

Previously published data demonstrate that the temporal processing algorithm for laser speckle contrast imaging (LSCI) can improve the visibility of deep blood vessels and is less susceptible to static speckle artifacts when compared with the spatial algorithm. To the best of our knowledge, the exte...

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Bibliographic Details
Published inJournal of biomedical optics Vol. 19; no. 10; p. 106009
Main Authors Ramirez-San-Juan, Julio C, Regan, Caitlin, Coyotl-Ocelotl, Beatriz, Choi, Bernard
Format Journal Article
LanguageEnglish
Published United States Society of Photo-Optical Instrumentation Engineers 01.10.2014
Subjects
Adult
Algorithms
Blood flow
Blood vessels
Camera tubes
Humans
Imaging
Lasers
Optical Imaging - methods
Phantoms, Imaging
Research Papers: Imaging
Scattering
Skin - chemistry
Speckle patterns
Temporal logic
Tooth - chemistry
laser speckle contrast imaging
laser speckle imaging
laser Doppler
blood flow measurement
biomedical imaging
laser speckle contrast analysis
microcirculation
microvasculature
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Summary:Previously published data demonstrate that the temporal processing algorithm for laser speckle contrast imaging (LSCI) can improve the visibility of deep blood vessels and is less susceptible to static speckle artifacts when compared with the spatial algorithm. To the best of our knowledge, the extent to which the temporal algorithm can accurately predict the speckle contrast associated with flow in deep blood vessels has not been quantified. Here, we employed two phantom systems and imaging setups (epi-illumination and transillumination) to study the contrast predicted by the spatial and temporal algorithms in subsurface capillary tubes as a function of the camera exposure time and the actual flow speed. Our data with both imaging setups suggest that the contrast predicted by the temporal algorithm, and therefore the relative flow speed, is nearly independent of the degree of static optical scattering that contributes to the overall measured speckle pattern. Collectively, these results strongly suggest the potential of temporal LSCI at a single-exposure time to assess accurately the changes in blood flow even in the presence of substantial static optical scattering.
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ISSN:1083-3668
1560-2281
DOI:10.1117/1.JBO.19.10.106009