Influence of phenolic acids on indole acetic acid production and on the type III secretion system gene transcription in food-associated Pseudomonas fluorescens KM05

• Published in: Journal of bioscience and bioengineering Vol. 118; no. 6; pp. 651 - 656
• Main Authors: Myszka, Kamila, Schmidt, Marcin T., Olejnik-Schmidt, Agnieszka K., Leja, Katarzyna, Czaczyk, Katarzyna
• Format: Journal Article
• Language: English
• Published: Amsterdam Elsevier B.V 01.12.2014; Elsevier
• Subjects: Bacterial Secretion Systems - drug effects; Bacterial Secretion Systems - genetics; Biological and medical sciences; Biotechnology; Chlorogenic acid; Chlorogenic Acid - pharmacology; Coumaric Acids - pharmacology; Ferulic acid; Food Microbiology; Fundamental and applied biological sciences. Psychology; Gallic acid; Gallic Acid - pharmacology; Gene Expression Regulation, Bacterial - drug effects; Gene Expression Regulation, Bacterial - genetics; Hydroxybenzoates - pharmacology; Indole acetic acid; Indoleacetic Acids - metabolism; Oxidation-Reduction; p-Coumaric acid; Propionates; Pseudomonas fluorescens; Pseudomonas fluorescens - drug effects; Pseudomonas fluorescens - genetics; Pseudomonas fluorescens - growth & development; Pseudomonas fluorescens - metabolism; Signal Transduction - drug effects; Transcription, Genetic - drug effects; Transcription, Genetic - genetics; Type III secretion system; Virulence - drug effects; Gallic acid; p-Coumaric acid; Indole acetic acid; Chlorogenic acid; Pseudomonas fluorescens; Type III secretion system; Ferulic acid; Pseudomonadales; Transcription; Phenolic acid; Indole; Foodstuff; Gene; Production; Bacteria; Pseudomonadaceae; Acetic acid
• ISSN: 1389-1723; 1347-4421
• DOI: 10.1016/j.jbiosc.2014.05.029

The purpose of these investigations was to evaluate the reduction capability of phenolic acids (ferulic, chlorogenic, gallic, and p-coumaric acids) on indole acetic acid synthesis by food-associated Pseudomonas fluorescens KM05. Specific genetic primer for the type III secretion system (TTSS) in P. fluorescens KM05 was designed and the influence of phenolic acids on its expression was investigated. In the work the ferulic and chlorogenic acids at the concentration of 0.02 and 0.04 μg/ml affected on bacterial growth pattern and the signal molecules production. The phenolic acids, that were appreciable effective against P. fluorescens KM05 indole acetic acid production, significantly suppressed TTSS gene. •Reduction effect of phenolics on indole acetic acid synthesis of cells was tested.•Specific genetic primer for TTSS system in P. fluorescens KM05 was designed.•Effect between indole acetic acid synthesis and TTSS gene expression was tested.•Ferulic and chlorogenic acids suppressed P. fluorescens KM05 signaling.•Ferulic and chlorogenic acids are promising interferers of bacterial virulence.