Drug Screening of Hair by Liquid Chromatography-Tandem Mass Spectrometry

• Published in: Journal of analytical toxicology Vol. 32; no. 5; pp. 364 - 372
• Main Authors: Hegstad, S., Khiabani, H.Z., Kristoffersen, L., Kunøe, N., Lobmaier, P.P., Christophersen, A.S.
• Format: Journal Article
• Language: English
• Published: Niles, IL Oxford University Press 01.06.2008; Preston
• Subjects: Analysis; Biological and medical sciences; Chromatography, High Pressure Liquid; Drug addictions; General pharmacology; Hair - chemistry; Humans; Medical sciences; Pharmacology. Drug treatments; Reference Standards; Reproducibility of Results; Spectrometry, Mass, Electrospray Ionization; Street Drugs - analysis; Substance Abuse Detection - methods; Tandem Mass Spectrometry - methods; Toxicology; Drug; Validation; Tandem mass spectrometry; Chemical analysis; Coupled method; Psychotropic; HPLC chromatography; Matrix effect; Medical screening; Electrospray; Biological material; Hair (head); Drug of abuse; Quantitative analysis
• ISSN: 0146-4760; 1945-2403
• DOI: 10.1093/jat/32.5.364

Hair has become an important matrix for drug analysis, complementary to blood and urine as a matrix. A prolonged detection window makes hair analysis suitable for lhe detection of exposure to illegal and medicinal drugs for periods up to 12 months. In the present study, a liquid chromatography-tandem mass spectrometry (LC-MS-MS) method for drug screening in hair was developed and validated. To 20 mg of hair, 0.45 mL of acetonitrile/25 mM formic acid (5:95 v/v) and 50 µL of deuterated internal standards were added, and the sample was incubated in a water bath at 37°C for 18 h. LC separation was achieved with a Zorbax SB-Phenyl column (2.1 × 100 mm, 3.5-µm particle). Mass detection was performed by positive ion mode electrospray LC-MS-MS and included the following drugs/metabolites: nicotine, cotinine, morphine, 6-monoacetylmorphine, codeine, amphetamine, methamphetamine, 3,4-methylenedioxymethamphetamine, cocaine, benzoylecgonine, 7-aminonitrazepam, 7-aminoclonazepam, 7-aminoflunitrazepam, oxazepam, diazepam, alprazolam, zopiclone, zolpidem, carisoprodol, meprobamate, buprenorphine, and methadone. Within- and between-assay relative standard deviations varied from 2.0% to 12% and 2.7% to 15%, respectively. The accuracies were in the range of −24% to 16%, and recoveries ranged from 25% to 100%. The LC-MS-MS method proved to be simple and robust for the determination of drugs in hair. It has been used for authentic samples in our laboratory in the past year.