Fecal Microbiota Restoration Modulates the Microbiome in Inflammation-Driven Colorectal Cancer

• Published in: Cancers Vol. 15; no. 8; p. 2260
• Main Authors: Gates, Travis J, Yuan, Ce, Shetty, Mihir, Kaiser, Thomas, Nelson, Andrew C, Chauhan, Aastha, Starr, Timothy K, Staley, Christopher, Subramanian, Subbaya
• Format: Journal Article
• Language: English
• Published: Switzerland MDPI AG 01.04.2023; MDPI
• Subjects: Akkermansia; Alistipes; Animals; Antibiotics; Azoxymethane; Bacteria; Bedding; Cancer; Care and treatment; Colitis; Colon cancer; Colorectal cancer; Colorectal carcinoma; Development and progression; Dextran; Drinking water; Fecal microflora; Feces; Health aspects; horizontal microbiota transfer; Inflammation; Inflammatory bowel disease; Intestinal microflora; Intestine; Lipocalin; longitudinal sampling; Metronidazole; microbiome; Microbiomes; Microbiota; Microbiota (Symbiotic organisms); Oncology, Experimental; Prevention; Risk factors; Sodium sulfate; Sulfates; Tumors; United States; Minnesota; colitis; colorectal cancer; horizontal microbiota transfer; microbiome; longitudinal sampling
• ISSN: 2072-6694; 2072-6694
• DOI: 10.3390/cancers15082260

Chronic inflammation of the colon (colitis) is a known risk factor for inflammatory-driven colorectal cancers (id-CRCs), and intestinal microbiota has been implicated in the etiology of id-CRCs. Manipulation of the microbiome is a clinically viable therapeutic approach to limiting id-CRCs. To understand the microbiome changes that occur over time in id-CRCs, we used a mouse model of id-CRCs with the treatment of azoxymethane (AOM) and dextran sodium sulfate (DSS) and measured the microbiome over time. We included cohorts where the microbiome was restored using cage bedding swapping and where the microbiome was depleted using antibiotics to compare to untreated animals. We identified consistent increases in in mice receiving horizontal microbiome transfer (HMT) via cage bedding swapping, while the control cohort had consistent longitudinal increases in and Additionally, fecal lipocalin-2 (Lcn-2), a marker of intestinal inflammation, was elevated in unrestored animals compared to restored and antibiotic-treated counterparts following HMT. These observations suggest a potential role for in regulating colonic inflammation in id-CRCs.