Analytical challenges and technical limitations in assessing circulating miRNAs

MiRNAs are emerging as promising biomarkers in cardiovascular diseases and may constitute a novel mechanism of intercellular communication. Accurate quantification of circulating miRNAs is essential. A variety of technological approaches and platforms have been developed with increased sensitivity a...

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Bibliographic Details
Published inThrombosis and haemostasis Vol. 108; no. 4; p. 592
Main Authors Zampetaki, Anna, Mayr, Manuel
Format Journal Article
LanguageEnglish
Published Germany 01.10.2012
Subjects
Blood Chemical Analysis - methods
DNA Primers - chemistry
DNA Primers - genetics
Gene Expression Profiling - methods
Humans
Microfluidic Analytical Techniques - methods
MicroRNAs - blood
MicroRNAs - chemistry
MicroRNAs - genetics
Molecular Probe Techniques
Nucleic Acid Conformation
Oligonucleotide Array Sequence Analysis - methods
Oligonucleotides
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Summary:MiRNAs are emerging as promising biomarkers in cardiovascular diseases and may constitute a novel mechanism of intercellular communication. Accurate quantification of circulating miRNAs is essential. A variety of technological approaches and platforms have been developed with increased sensitivity and specificity for the detection and quantification of circulating miRNAs. In this review, we focus on the technical aspects and discuss the analytical challenges in profiling circulating miRNAs.
ISSN:2567-689X
DOI:10.1160/TH12-02-0097