Anti-inflammatory and antiarthritic activity of UNIM-301 (a polyherbal unani formulation) in Wistar rats

• Published in: Pharmacognosy research Vol. 7; no. 2; pp. 188 - 192
• Main Authors: Singh, Surender, Kumar, Rohit, Jain, Hitesh, Gupta, Y
• Format: Journal Article
• Language: English
• Published: India Medknow Publications Pvt Ltd 01.04.2015; Medknow Publications and Media Pvt. Ltd; Phcog.net; Medknow Publications & Media Pvt Ltd
• Subjects: Albinism; Animals; Anti-inflammatory agents; Antiarthritic agents; Arthritis; Carrageenan; Cytokines; Disease; Drug dosages; Edema; Inflammation; Laboratories; Mortality; Original; Rheumatoid factor; Rodents; Tumor necrosis factor-TNF; UNIM-301; carrageenan-induced paw edema; Adjuvant arthritis
• ISSN: 0974-8490; 0976-4836; 0974-8490
• DOI: 10.4103/0974-8490.150515

Background: UNIM-301 is a polyherbal formulation used in the Unani system of medicine for the treatment of joint pain and rheumatoid arthritis (RA). Objective: The objective was to evaluate the anti-inflammatory and antiarthritic activity of UNIM-301 in carrageenan-induced paw edema and complete Freund′s Adjuvant (CFA) induced arthritis. Materials and Methods: The anti-inflammatory and antiarthritic activity of UNIM-301 was evaluated using carrageenan-induced paw edema and CFA induced animal arthritis models, respectively, in doses of 250, 500, and 1000 mg/kg body weight. Anti-inflammatory activity of UNIM-301 was evaluated using carrageenan-induced paw edema model using a digital plethysmometer. Anti-arthritic activity was evaluated using CFA induced arthritis, and joint sizes were measured at regular intervals using a micrometer screw gauge. Serum was collected and subjected to estimation of pro-inflammatory cytokine. Indomethacin 3 mg/kg body weight) was used as a standard drug in both the models. The acute and chronic toxicity study was carried out to evaluate the safety of the test drug. Results: UNIM-301 treatment produced a dose-dependent reduction in paw edema and paw thickness in carrageenan-induced paw edema and CFA-induced arthritis, respectively, as compared to control. UNIM 301 also reduced the expression of pro-inflammatory mediator in a dose-dependent manner as compared to control. Conclusion: The result of the present study suggests that anti-inflammatory and anti-arthritic activity of UNIM-301, which might be accredited to inhibitory activity on pro-inflammatory cytokines to its various individual constituents.