OsHMA2 transporter is involved in root‐to‐shoot translocation of Zn and Cd in rice

• Published in: Plant, cell and environment Vol. 35; no. 11; pp. 1948 - 1957
• Main Authors: TAKAHASHI, RYUICHI, ISHIMARU, YASUHIRO, SHIMO, HUGO, OGO, YUKO, SENOURA, TAKESHI, NISHIZAWA, NAOKO K, NAKANISHI, HIROMI
• Format: Journal Article
• Language: English
• Published: Oxford, UK Blackwell Publishing Ltd 01.11.2012; Blackwell; Wiley Subscription Services, Inc
• Subjects: Biological and medical sciences; Biological Transport; cadmium; Cadmium - metabolism; Cation Transport Proteins - analysis; Cation Transport Proteins - genetics; Cation Transport Proteins - physiology; food safety; Fundamental and applied biological sciences. Psychology; Green Fluorescent Proteins - analysis; heavy metal ATPase; heavy metals; humans; leaves; metal transporter; onions; Oryza - genetics; Oryza - metabolism; Oryza sativa; phenotype; Plant Leaves - metabolism; Plant Proteins - analysis; Plant Proteins - genetics; Plant Proteins - physiology; Plant Roots - metabolism; Plant Shoots - metabolism; plasma membrane; Recombinant Fusion Proteins - analysis; rice; roots; vascular bundles; xylem; yeasts; zinc; Zinc - metabolism; Translocation; Cadmium; Monocotyledones; Metallophore; Root; Enzyme; Adenosinetriphosphatase; Plant ecology; heavy metal ATPase; Cereal crop; Zinc; Heavy metal; metal transporter; Oryza sativa; Gramineae; Angiospermae; Hydrolases; Spermatophyta
• ISSN: 0140-7791; 1365-3040
• DOI: 10.1111/j.1365-3040.2012.02527.x

Zinc (Zn) is an essential micronutrient for plants and humans. Cadmium (Cd) is a Zn analog and one of the most toxic heavy metals to humans. Here we investigated the role of the Zn/Cd transporter OsHMA2. OsHMA2:GFP fusion protein localized to the plasma membrane in onion epidermal cells. The yeast expressing OsHMA2 was able to reverse the growth defect in the presence of excess Zn. The expression of OsHMA2 in rice was observed mainly in the roots where OsHMA2 transcripts were abundant in vascular bundles. Furthermore, Zn and Cd concentrations of OsHMA2‐suppressed rice decreased in the leaves, while the Zn concentration increased in the roots compared with the wild type (WT). These results suggest that OsHMA2 plays a role in Zn and Cd loading to the xylem and participates in root‐to‐shoot translocation of these metals in rice. Furthermore, the Cd concentration in the grains of OsHMA2‐overexpressing rice as well as in OsSUT1‐promoter OsHMA2 rice decreased to about half that of the WT, although the other metal concentrations were the same as in the WT. A phenotype that reduces only the Cd concentration in rice grains will be very useful for transgenic approaches to food safety.