Anaphylaxis to camomile: clinical features and allergen cross-reactivity

• Published in: Clinical and experimental allergy Vol. 30; no. 10; pp. 1436 - 1443
• Main Authors: Reider, N., Sepp, N., Fritsch, P., Weinlich, G., Jensen-Jarolim, E.
• Format: Journal Article
• Language: English
• Published: Oxford BSL Blackwell Science Ltd 01.10.2000; Blackwell; Wiley Subscription Services, Inc
• Subjects: Adolescent; Adult; Aged; Allergens - immunology; Allergic diseases; allergy; anaphylaxis; Anaphylaxis - etiology; Animals; Anthemis nobilis; Bet v 1; Biological and medical sciences; camomile; carbohydrate; Chamomile - immunology; Cross Reactions; cross-reactivity; Female; General aspects; Glycosylation; higher molecular weight allergens; Humans; IgE; Immunoblotting; Immunoglobulin E - immunology; Immunopathology; Male; Medical sciences; Middle Aged; mugwort-celery-spice-syndrome; Plant Extracts - immunology; Plants, Medicinal; Pollen - immunology; profilin; Rabbits; Human; Immunopathology; Allergy; Specific IgE; IgE; Medicinal plant; Symptomatology; Crossed allergy; Anaphylaxis; Immunoblotting assay; Diagnosis; Chamomile; Allergen; Skin test
• ISSN: 0954-7894; 1365-2222
• DOI: 10.1046/j.1365-2222.2000.00902.x

Background Medicinal remedies of plant origin became very popular in recent years, and allergic reactions to these are on the rise, accordingly. Camomile has been reported as a potential trigger of severe anaphylaxis. The allergens responsible for camomile allergy have not been characterized as yet. Objective The present study aims at reviewing the clinical symptomatology of immediate‐type reactions in a series of patients sensitized to camomile and at characterizing the responsible allergens. Methods Fourteen patients with a history of allergy either to camomile or to spices or weeds, and a positive skin prick test/RAST to camomile were investigated for related allergic reactions to food, pollen and others. IgE‐binding patterns were determined by immunoblotting, inhibition tests and deglycosylation experiments. Results Ten of 14 patients had a clinical history of immediate‐type reactions to camomile, in some cases life threatening. Eleven subjects were also sensitized to mugwort in prick or RAST, eight to birch tree pollen. Using a polyclonal rabbit anti‐Bet v 1 antibody, a homologue of the major birch pollen allergen Bet v 1 was detected in two camomile blots. In four cases a group of higher molecular weight allergens (23–50 kDa) showed IgE‐binding to camomile. All allergens proved heat stable. Binding was inhibited in variable degrees by extracts from celery roots, anize seeds and pollen from mugwort, birch and timothy grass. Deglycosylation experiments proved the presence of carbohydrate determinants in camomile which were not responsible for IgE‐binding, though. Profilins (Bet v 2) were not detected in our camomile extracts. Conclusion Incidence and risk of type I allergy to camomile may be underestimated. Concurrent sensitization to mugwort and birch pollen is not infrequent. Bet v 1 and noncarbohydrate higher molecular weight proteins were found to be eliciting allergens and are responsible for cross‐reactivity with other foods and pollen.