IMPACT OF IMMUNE RESPONSE OF A PARASITIC BEETLE Dastarcus helophoroides ON ITS HOST BEETLE Monochamus alternatus

• Published in: Archives of insect biochemistry and physiology Vol. 90; no. 1; pp. 28 - 42
• Main Authors: Li, Xiao‐juan, Dong, Guang‐ping, Fang, Jian‐min, Liu, Hong‐jian, Yang, Li, Guo, Wan‐lin
• Format: Journal Article
• Language: English
• Published: United States Alan R. Liss 01.09.2015; Blackwell Publishing Ltd; Wiley Subscription Services, Inc
• Subjects: Animals; antibacterial properties; Coleoptera - enzymology; Coleoptera - immunology; Coleoptera - parasitology; Dastarcus helophoroides; Hemagglutination; hemocytes; Hemocytes - immunology; Hemolymph - enzymology; Hemolymph - immunology; Host-Parasite Interactions; hosts; Immune response; Immune system; Larva - enzymology; Larva - immunology; Larva - parasitology; Larvae; melanization; Monochamus alternatus; monophenol monooxygenase; nodulation; Parasitism; Dastarcus helophoroides; immune response; Monochamus alternatus
• ISSN: 0739-4462; 1520-6327; 1520-6327
• DOI: 10.1002/arch.21242

Dastarcus helophoroides is an ectoparasitoid beetle of Monochamus alternatus, and the parasitism by D. helophoroides larvae remarkably influenced on the immune responses of M. alternatus larvae in many aspects. The hemolymph melanization reactions in the hosts were inhibited 1 h and 24 h postparasitization. The phenoloxidase activities of hemolymph were significantly stimulated 4 h postparasitization and inhibited 12 h postparasitization, and back to control level. The antibacterial activities of hemolymph in the parasitized hosts were significantly lower than that in the unparasitized ones 1 h postparasitization. By 72 h postparasitism, the total hemocyte numbers of the parasitized larvae declined to not more than one‐seconds of the number collected from the unparasitized larvae. All sampled hemolymph held the capability of nodulation, and there were fluctuations in the number of nodules the hemocytes made. However, there were no significant differences between unparasitized and parasitized larvae at each time point in the hemagglutination activity and the ratios of spreading hemocytes. In conclusion, D. helophoroides larvae could regulate M. alternatus immune system and resulted in the changes in host immune responses.