STGC3 inhibits xenograft tumor growth of nasopharyngeal carcinoma cells by altering the expression of proteins associated with apoptosis

STGC3 is a potential tumor suppressor that inhibits the growth of the nasopharyngeal carcinoma cell line CNE2; the expression of this protein is reduced in nasopharyngeal carcinoma compared with normal nasopharyngeal tissue. In this study, we investigated the tumor-suppressing activity of STGC3 in n...

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Published inGenetics and molecular biology Vol. 35; no. 1; pp. 18 - 26
Main Authors Qiu, Qing-Chao, Hu, Bo, He, Xiu-Pei, Luo, Qiao, Tang, Guo-Hua, Long, Zhi-Feng, Chen, Zhu-Chu, He, Xiu-Sheng
Format Journal Article
LanguageEnglish
Portuguese
Published Brazil Sociedade Brasileira de Genética 01.01.2012
Subjects
BIOCHEMISTRY & MOLECULAR BIOLOGY
CNE2 cell line
GENETICS & HEREDITY
nasopharyngeal carcinoma
nude mouse
STGC3
Tet-on
two-dimensional electrophoresis
CNE2 cell line
Tet-on
nasopharyngeal carcinoma
nude mouse
two-dimensional electrophoresis
STGC3
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Summary:STGC3 is a potential tumor suppressor that inhibits the growth of the nasopharyngeal carcinoma cell line CNE2; the expression of this protein is reduced in nasopharyngeal carcinoma compared with normal nasopharyngeal tissue. In this study, we investigated the tumor-suppressing activity of STGC3 in nude mice injected subcutaneously with Tet/pTRE-STGC3/CNE2 cells. STGC3 expression was induced by the intraperitoneal injection of doxycycline (Dox). The volume mean of Tet/pTRE-STGC3/CNE2+Dox xenografts was smaller than that of Tet/pTRE/CNE2+Dox xenografts. In addition, Tet/pTRE-STGC3/CNE2+Dox xenografts showed an increase in the percentage of apoptotic cells, a decrease in Bcl-2 protein expression and an increase in Bax protein expression. A proteomic approach was used to assess the protein expression profile associated with STGC3-mediated apoptosis. Western blotting confirmed the differential up-regulation of prohibitin seen in proteomic analysis. These results indicate that overexpression of STGC3 inhibits xenograft growth in nude mice by enhancing apoptotic cell death through altered expression of apoptosis-related proteins such as Bcl-2, Bax and prohibitin. These data contribute to our understanding of the function of STGC3 in human nasopharyngeal carcinoma and provide new clues for investigating other STGC3-associated tumors.
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These authors contributed equally to this work.
Associate Editor: Emmanuel Dias Neto
ISSN:1415-4757
1678-4685
1678-4685
DOI:10.1590/S1415-47572012005000009