Expression of pituitary prolactin, growth hormone and somatolactin is modified in response to different stressors (salinity, crowding and food-deprivation) in gilthead sea bream Sparus auratus

• Published in: General and comparative endocrinology Vol. 162; no. 3; pp. 293 - 300
• Main Authors: Laiz-Carrión, Raúl, Fuentes, Juan, Redruello, Begoña, Guzmán, José M, Martín del Río, María P, Power, Deborah, Mancera, Juan M
• Format: Journal Article
• Language: English
• Published: United States Elsevier Inc 01.07.2009
• Subjects: Animals; Blood Glucose - physiology; Brackish; Chlorides - blood; Endocrinology & Metabolism; Fish Proteins - genetics; Food deprivation; Food Deprivation - physiology; Gene Expression Regulation; Gills - metabolism; Glycoproteins - genetics; Growth Hormone - genetics; Hydrocortisone - blood; Marine; Osmolar Concentration; Osmoregulation; Pituitary Gland - metabolism; Pituitary Hormones - genetics; Population Density; PRL; Prolactin - genetics; Salinity; Sea bream; Sea Bream - genetics; Sea Bream - physiology; Sodium - blood; Sodium-Potassium-Exchanging ATPase - metabolism; Sparus aurata; Sparus auratus; Stocking density; Stress, Physiological - genetics; GH; Food deprivation; Sparus auratus; Stocking density; Osmoregulation; Sea bream; SL; PRL
• ISSN: 0016-6480; 1095-6840
• DOI: 10.1016/j.ygcen.2009.03.026

Abstract Prolactin (PRL), growth hormone (GH) and somatolactin (SL) expression was studied in gilthead sea bream ( Sparus auratus ) in response to several different stressors (salinity, food deprivation or stocking density). In the first experiment, specimens were acclimated during 100 days at three different environmental salinities: low salinity water (LSW, 6 ppt), brackish water (BW, 12 ppt) and seawater (SW, 38 ppt). Osmoregulatory parameters corresponded to those previously reported for this species under similar osmotic conditions. Pituitary PRL expression increased with decreasing environmental salinity, and was significantly different between SW- and LSW-acclimated fish. Pituitary GH expression was similar between SW- and BW-acclimated fish but decreased in LSW-acclimated specimens. Pituitary SL expression had a “U-shaped” relationship to environmental salinity with the lowest expression in BW-acclimated fish. In a second experiment SW-acclimated specimens were randomly assigned to one of four treatments and maintained for 14 days: (1) fed fish under low density (LD, 4 kg m−3 ); (2) fed fish under high density (HD, 70 kg m−3 ); (3) food deprived fish under LD; and (4) food deprived fish under HD. Plasma glucose and cortisol levels corresponded to those previously reported in S. auratus under similar experimental conditions. Pituitary PRL and SL expression increased in fish maintained under HD and decreased in food deprived fish. In conclusion, an effect of environmental salinity on pituitary PRL and GH expression has been demonstrated. In addition, crowding stress seems to interact with food deprivation in S. auratus and this is reflected by changes in pituitary PRL, GH and SL expression levels.