CRISPR-Based Technologies for the Manipulation of Eukaryotic Genomes

• Published in: Cell Vol. 168; no. 1-2; pp. 20 - 36
• Main Authors: Komor, Alexis C., Badran, Ahmed H., Liu, David R.
• Format: Journal Article
• Language: English
• Published: United States Elsevier Inc 12.01.2017
• Subjects: Animals; biotechnology; Cells, Cultured; CRISPR-Cas Systems; Disease - genetics; Disease Models, Animal; DNA; Epigenomics - methods; Gene Editing - methods; Genetic Therapy; genome; Humans; mammals; therapeutics

The CRISPR-Cas9 RNA-guided DNA endonuclease has contributed to an explosion of advances in the life sciences that have grown from the ability to edit genomes within living cells. In this Review, we summarize CRISPR-based technologies that enable mammalian genome editing and their various application...