CRISPR-Based Technologies for the Manipulation of Eukaryotic Genomes

The CRISPR-Cas9 RNA-guided DNA endonuclease has contributed to an explosion of advances in the life sciences that have grown from the ability to edit genomes within living cells. In this Review, we summarize CRISPR-based technologies that enable mammalian genome editing and their various application...

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Bibliographic Details
Published inCell Vol. 168; no. 1-2; pp. 20 - 36
Main Authors Komor, Alexis C., Badran, Ahmed H., Liu, David R.
Format Journal Article
LanguageEnglish
Published United States Elsevier Inc 12.01.2017
Subjects
Animals
biotechnology
Cells, Cultured
CRISPR-Cas Systems
Disease - genetics
Disease Models, Animal
DNA
Epigenomics - methods
Gene Editing - methods
Genetic Therapy
genome
Humans
mammals
therapeutics
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Summary:The CRISPR-Cas9 RNA-guided DNA endonuclease has contributed to an explosion of advances in the life sciences that have grown from the ability to edit genomes within living cells. In this Review, we summarize CRISPR-based technologies that enable mammalian genome editing and their various applications. We describe recent developments that extend the generality, DNA specificity, product selectivity, and fundamental capabilities of natural CRISPR systems, and we highlight some of the remarkable advancements in basic research, biotechnology, and therapeutics science that these developments have facilitated. CRISPR-based genome-editing technologies provide powerful tools to study basic biology and may lead to new treatments for human disease.
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ISSN:0092-8674
1097-4172
1097-4172
DOI:10.1016/j.cell.2016.10.044