Establishment of a CD4-positive cell line from an AIDS-related primary effusion lymphoma

• Published in: International journal of hematology Vol. 97; no. 5; pp. 624 - 633
• Main Authors: Goto, Hiroki, Kojima, Yuki, Nagai, Hirokazu, Okada, Seiji
• Format: Journal Article
• Language: English
• Published: Japan Springer Japan 01.05.2013; Springer; Springer Nature B.V
• Subjects: Animals; Biological and medical sciences; CD4 Antigens - metabolism; Cell Line, Tumor; Chromosome Aberrations; Chromosome Banding; Chylous Ascites; Epstein-Barr virus; Hematologic and hematopoietic diseases; Hematology; Herpesvirus 4, Human - genetics; Herpesvirus 8, Human - genetics; HHV-8; HIV-1; Human herpesvirus 8; Human immunodeficiency virus 1; Human viral diseases; Humans; Immunophenotyping; Infectious diseases; Leukemias. Malignant lymphomas. Malignant reticulosis. Myelofibrosis; Lymphoma, AIDS-Related - genetics; Lymphoma, AIDS-Related - metabolism; Lymphoma, AIDS-Related - pathology; Lymphoma, Primary Effusion - genetics; Lymphoma, Primary Effusion - metabolism; Lymphoma, Primary Effusion - pathology; Medical sciences; Medicine; Medicine & Public Health; Mice; Mouse model; Oncology; Original Article; Primary effusion lymphoma; Transplantation, Heterologous; Viral diseases; Viral diseases of the lymphoid tissue and the blood. Aids; HIV-1; HHV-8; Mouse model; Primary effusion lymphoma; Animal model; CD4 T lymphocyte; HIV-1 virus; Human herpesvirus 8; Lymphoproliferative syndrome; Established cell line; Gammaherpesvirinae; Immunopathology; Hematology; Herpesviridae; Rodentia; Retroviridae; AIDS; B cell neoplasm; Malignant hemopathy; Non Hodgkin lymphoma; Immune deficiency; Lentivirus; Infection; Virus; Vertebrata; Mammalia; Mouse; Viral disease; Human immunodeficiency virus; Cancer
• ISSN: 0925-5710; 1865-3774; 1865-3774
• DOI: 10.1007/s12185-013-1339-3

Primary effusion lymphoma (PEL) presents as a serous lymphomatous effusion without tumor masses exclusively in body cavities and mainly occurs in human immunodeficiency virus-1 (HIV-1)-infected patients. We established a new PEL cell line, designated GTO, from the pericardial effusion of a 39-year-old Japanese patient with acquired immunodeficiency syndrome-related PEL. This cell line was infected with human herpesvirus-8, but not with Epstein–Barr virus. Southern blot hybridization demonstrated that GTO cells display monoclonal rearrangement of the IgH gene, suggesting clonal B cell proliferation. GTO cells weakly express or lack T cell-associated markers (CD3, CD5, CD8), the majority of B cell-associated markers (CD19, CD20, CD21, CD79a), the α chains of β 2 integrins (CD11a, CD11b, CD11c), HLA-DR, CD30, and surface immunoglobulin (sIgM, sIgG sIgκ, sIgλ), TCR (α/β, γδ), but express CD45, and post-germinal center B cell/plasma cell-associated antigens (CD38, CD138). They also express a high level of cell-surface CD4 and can be infected by HIV-1. Immunodeficient mice intraperitoneally xenografted with GTO cells developed ascites containing lymphoma cells. The establishment of GTO and a GTO xenograft mouse model may help to provide insights toward a better understanding of the pathogenesis of PEL and the relationship between HIV-1 and HHV-8.