Isolation and characterization of alkalotolerant Pseudomonas sp. strain ISTDF1 for degradation of dibenzofuran

• Published in: Journal of industrial microbiology & biotechnology Vol. 38; no. 4; pp. 503 - 511
• Main Authors: Jaiswal, Prashant Kumar, Kohli, Shweta, Gopal, Madhuban, Thakur, Indu Shekhar
• Format: Journal Article
• Language: English
• Published: Berlin/Heidelberg Berlin/Heidelberg : Springer-Verlag 01.04.2011; Springer-Verlag; Springer; Springer Nature B.V
• Subjects: Acids; Analysis; Bacteria; Benzofurans - metabolism; Biochemistry; Biodegradation; Biodegradation, Environmental; Bioinformatics; Biological and medical sciences; Biomedical and Life Sciences; Bioremediation; Biotechnology; Carbon; dibenzofuran; Dioxins; Dioxins - metabolism; energy; environmental factors; Fundamental and applied biological sciences. Psychology; Gas Chromatography-Mass Spectrometry; Genetic Engineering; Hydrogen-Ion Concentration; Inorganic Chemistry; Life Sciences; Metabolites; Microbiology; Morphology; Original Paper; Pollutants; polycyclic aromatic hydrocarbons; Pseudomonas; Pseudomonas - growth & development; Pseudomonas - isolation & purification; Pseudomonas - metabolism; Pulp & paper industry; pulp and paper industry; Scanning electron microscopy; Studies; temperature; Thermal cycling; Biodegradation; sp. strain ISTDF1; GC–MS; Dibenzofuran; Alkalotolerant; Pseudomonadales; Characterization; GC-MS; Bacteria; Pseudomonadaceae; Isolation; Pseudomonas sp; strain ISTDF1; Pseudomonas
• ISSN: 1367-5435; 1476-5535
• DOI: 10.1007/s10295-010-0793-7

An alkalotolerant Pseudomonas strain was enriched and isolated from effluent of the pulp and paper industry. This strain was able to degrade dibenzofuran and utilize it as a sole source of energy and carbon. The GC-MS based detection of various intermediary metabolites of biodegradation suggested the involvement of angular as well as lateral pathway of dibenzofuran biodegradation. The GC-MS based detection of various intermediary metabolites of biodegradation suggested the involvement of angular as well as lateral pathway of dibenzofuran biodegradation. This diverse dioxygenation property of the strain allowed it to utilize various recalcitrant chlorinated xenobiotics and PAHs compounds. This strain showed optimum utilization (~85%) of dibenzofuran (200 mg l⁻¹) within 36 h at pH 10 at 40°C. The growth of the strain was supported by a wide range of environmental conditions such as temperature, pH, and concentration of dibenzofuran, suggesting that it can be used for in situ bioremediation of dioxin-like compound.