Functional Analysis of the Twin-Arginine Translocation Pathway in Sodalis glossinidius, a Bacterial Symbiont of the Tsetse Fly

• Published in: Applied and Environmental Microbiology Vol. 77; no. 3; pp. 1132 - 1134
• Main Authors: De Vooght, Linda, Caljon, Guy, Coosemans, Marc, Van Den Abbeele, Jan
• Format: Journal Article
• Language: English
• Published: Washington, DC American Society for Microbiology 01.02.2011; American Society for Microbiology (ASM)
• Subjects: Animals; Bacterial Proteins - genetics; Bacterial Proteins - metabolism; Biological and medical sciences; Enterobacteriaceae - classification; Enterobacteriaceae - genetics; Enterobacteriaceae - metabolism; Escherichia coli; Escherichia coli Proteins; Fluorescence; Fundamental and applied biological sciences. Psychology; Glossina; Green Fluorescent Proteins - genetics; Green Fluorescent Proteins - metabolism; Insects; Invertebrate Microbiology; Membrane Transport Proteins - genetics; Membrane Transport Proteins - metabolism; Microbiology; Oxidoreductases, N-Demethylating - genetics; Oxidoreductases, N-Demethylating - metabolism; Peptides; Proteins; Signal transduction; Sodalis glossinidius; Symbiosis; Tsetse Flies - microbiology; Translocation; Bacteria; Functional analysis; Arginine; Symbiont
• ISSN: 0099-2240; 1098-5336; 1098-6596
• DOI: 10.1128/AEM.02379-10

This study demonstrates a functional twin-arginine (Tat) translocation pathway present in the tsetse fly symbiont Sodalis glossinidius and its potential to export active heterologous proteins to the periplasm. Functionality was demonstrated using green fluorescent protein (GFP) fused to the Tat signal peptide of Escherichia coli trimethylamine N-oxide reductase (TorA).