Clonal analysis of human dendritic cell progenitor using a stromal cell culture

• Published in: Journal of immunological methods Vol. 425; pp. 21 - 26
• Main Authors: Lee, Jaeyop, Breton, Gaëlle, Aljoufi, Arafat, Zhou, Yu Jerry, Puhr, Sarah, Nussenzweig, Michel C., Liu, Kang
• Format: Journal Article
• Language: English
• Published: Netherlands Elsevier B.V 01.10.2015
• Subjects: Antigens, CD34 - immunology; Bone Marrow - immunology; Cell Differentiation - immunology; Cell Lineage - immunology; Cells, Cultured; Culture; Dendritic cell; Dendritic Cells - immunology; Granulocyte-Macrophage Progenitor Cells - immunology; Granulocyte–macrophage progenitor; Hematopoiesis; Hematopoiesis - immunology; Humans; Lymphocytes - immunology; Single cell; Stem Cells - immunology; Stromal cells; Stromal Cells - immunology; Single cell; Dendritic cell; Hematopoiesis; Stromal cells; Culture; Granulocyte–macrophage progenitor
• ISSN: 0022-1759; 1872-7905
• DOI: 10.1016/j.jim.2015.06.004

Different dendritic cell (DC) subsets co-exist in humans and coordinate the immune response. Having a short life, DCs must be constantly replenished from their progenitors in the bone marrow through hematopoiesis. Identification of a DC-restricted progenitor in mouse has improved our understanding of how DC lineage diverges from myeloid and lymphoid lineages. However, identification of the DC-restricted progenitor in humans has not been possible because a system that simultaneously nurtures differentiation of human DCs, myeloid and lymphoid cells, is lacking. Here we report a cytokine and stromal cell culture that allows evaluation of CD34+ progenitor potential to all three DC subsets as well as other myeloid and lymphoid cells, at a single cell level. Using this system, we show that human granulocyte–macrophage progenitors are heterogeneous and contain restricted progenitors to DCs.