Detection of Adherence of Enterococcus faecalis in Infected Dentin of Extracted Human Teeth Using Confocal Laser Scanning Microscope: An In vitro Study

• Published in: Journal of pharmacy & bioallied science Vol. 9; no. Suppl 1; pp. S41 - S44
• Main Authors: Nair, V Siddharth, Nayak, Moksha, Ramya, M K, Sivadas, G, Ganesh, C, Devi, S Lakshmi, Vedam, Vaishnavi
• Format: Journal Article
• Language: English
• Published: India Medknow Publications and Media Pvt. Ltd 01.11.2017; Medknow Publications & Media Pvt. Ltd; Medknow Publications & Media Pvt Ltd; Wolters Kluwer Medknow Publications
• Subjects: Acridine orange; Adherence; American Type Culture Collection; Bacteria; Bacterial proteins; Biofilms; confocal laser scanning microscopy; Confocal microscopy; Dental roots; Dentin; Dentistry; Disease; Dyes; Endodontics; Enterococcal infections; Enterococcus faecalis; Fluorescein diacetate; Infections; Lasers; Metabolism; Microscope and microscopy; Microscopes; Microscopy; Original; Polymerase chain reaction; Root canals; Teeth; Tubules; Adherence; Enterococcus faecalis; confocal laser scanning microscopy
• ISSN: 0976-4879; 0975-7406; 0975-7406
• DOI: 10.4103/jpbs.JPBS_92_17

The aim of this study is to detect adherence of to the infected dentinal tubules of human extracted teeth using confocal laser scanning microscope. Roots from human premolar teeth ( = 40) were infected with strain the American Type Culture Collection 29212 in brain heart infusion for 21 days. After the experimental period, specimens were divided into two groups, Group A ( = 20), Group B ( = 20), and Group A specimens were stained with fluorescein diacetate dye for the detection of viability and adherence Group B were stained with acridine orange dye for detection of metabolic activity and adherence. Samples were washed, thoroughly sectioned and examined by confocal laser scanning microscopy. Computer-assisted determinants of fluorescence, bacterial viability, metabolic activity, and adherence were compared statistically. was able to invade the dentinal tubules to a depth of 1-400 μm and adhere to 1-200 μm depth. Adherence (90%) was significantly higher in 1-100 μm using fluorescein diacetate and acridine orange dye. Adherence of as evaluated by confocal laser scanning microscope was highest at the depth of 1-100 μm which may have an impact on the shaping and cleaning procedures on the root canal.