Overexpression of TaPIEP1, a pathogen-induced ERF gene of wheat, confers host-enhanced resistance to fungal pathogen Bipolaris sorokiniana

• Published in: Functional & integrative genomics Vol. 10; no. 2; pp. 215 - 226
• Main Authors: Dong, Na, Liu, Xin, Lu, Yan, Du, LiPu, Xu, Huijun, Liu, Hongxia, Xin, Zhiyong, Zhang, Zengyan
• Format: Journal Article
• Language: English
• Published: Berlin/Heidelberg Berlin/Heidelberg : Springer-Verlag 01.05.2010; Springer-Verlag; Springer; Springer Nature B.V
• Subjects: Abscisic acid; Amino Acid Sequence; Animal Genetics and Genomics; Ascomycota; Ascomycota - drug effects; Ascomycota - physiology; Base Sequence; Biochemistry; Bioinformatics; biolistics; Biological and medical sciences; Biomedical and Life Sciences; Bipolaris sorokiniana; Cell Biology; Cell Nucleus; Cell Nucleus - drug effects; Cell Nucleus - metabolism; Cereals; chemistry; Cochliobolus sativus; Crops; Disease resistance; drug effects; Ethylene; Ethylene-responsive factor (ERF); Fundamental and applied biological sciences. Psychology; Gene expression; Gene Expression Regulation, Plant; Gene Expression Regulation, Plant - drug effects; gene overexpression; General aspects; genes; Genes, Plant; Genes, Plant - genetics; genetics; Genomics; Host-Pathogen Interactions; Host-Pathogen Interactions - drug effects; Host-Pathogen Interactions - genetics; host-pathogen relationships; Immunity, Innate; Immunity, Innate - drug effects; Immunity, Innate - genetics; immunology; Jasmonic acid; Life Sciences; Mathematics in biology. Statistical analysis. Models. Metrology. Data processing in biology (general aspects); metabolism; Microbial Genetics and Genomics; microbiology; Molecular Sequence Data; Original Paper; Pathogens; pharmacology; Phenotype; Phylogeny; physiology; Plant Diseases; Plant Diseases - genetics; Plant Diseases - immunology; Plant Diseases - microbiology; Plant Genetics and Genomics; Plant Growth Regulators; Plant Growth Regulators - pharmacology; plant pathogenic fungi; Plant pathology; Plant Proteins; Plant Proteins - chemistry; Plant Proteins - genetics; Plant Proteins - metabolism; Plant resistance; Plants, Genetically Modified; Protein Binding; Protein Binding - drug effects; Protein Transport; Protein Transport - drug effects; Regulatory Sequences, Nucleic Acid; Regulatory Sequences, Nucleic Acid - genetics; Reverse Transcriptase Polymerase Chain Reaction; screening; Sequence Analysis, DNA; Trans-Activators; Trans-Activators - chemistry; Trans-Activators - genetics; Trans-Activators - metabolism; transactivators; Transcription; Transcription factors; Transcription, Genetic; Transcription, Genetic - drug effects; Transgenic plants; Transgenic wheat; Triticum; Triticum - drug effects; Triticum - genetics; Triticum - immunology; Triticum - microbiology; Triticum aestivum; Wheat; Ethylene-responsive factor (ERF); Disease resistance; Transgenic wheat; Monocotyledones; Disease; Ethylene; Fungi; Resistance; Gene; Gramineae; Angiospermae; Cereal; Spermatophyta; Fungi Imperfecti; Bipolaris sorokiniana; Wheat; Triticum aestivum
• ISSN: 1438-793X; 1438-7948; 1438-7948
• DOI: 10.1007/s10142-009-0157-4

Bipolaris sorokiniana is an economically important phytopathogen of wheat and other cereal species. In this paper, a novel pathogen-induced ethylene-responsive factor (ERF) gene of wheat, TaPIEP1, was isolated and characterized. The transcript of TaPIEP1 was significantly and rapidly induced by treatments with B. sorokiniana, and with ethylene (ET), jasmonate (JA), and abscisic acid. Molecular and biochemical assays demonstrated that TaPIEP1 is a new ERF transcription activator belonging to B-3c subgroup of the ERF family. Transgenic wheat lines overexpressing TaPIEP1 were generated by biolistic bombardment and molecular screening. Compared with the host wheat Yangmai12, six stable transgenic wheat lines overexpressing TaPIEP1 that exhibited significantly increased resistance to B. sorokiniana were identified by molecular detection in the T₀-T₄ generations and by disease resistance tests. The degree of the enhanced resistance was correlated with an accumulation of the transcript level of TaPIEP1. Furthermore, the transcript levels of certain defense-related genes in the ET/JA pathways were markedly increased in the transgenic wheat plants with enhanced resistance. These results reveal that TaPIEP1 overexpression in wheat could obviously improve resistance to B. sorokiniana via activation of some defense genes, and TaPIEP1 gene may be useful in improving crop resistance to the pathogen.