The Biological Significance of Glycyrrhizin- and Glycyrrhetinic Acid Derivative-Induced Selective Phosphorylation of Histones H2A and H2B by A-kinase In Vitro

• Published in: The Tohoku Journal of Experimental Medicine Vol. 172; no. 2; pp. 123 - 132
• Main Authors: SHAMSA, FAZEL, IWASA, TAKESHI, SAITO, HITOSHI, NAGATA, NOBUYUKI, OHTSUKI, KENZO
• Format: Journal Article
• Language: English
• Published: Japan Tohoku University Medical Press 1994
• Subjects: A-kinase; Animals; Anti-Infective Agents - pharmacology; anti-inflammatory effect; Carcinoma, Ehrlich Tumor - enzymology; Cattle; Chromatography, Affinity; DNA - pharmacology; Dose-Response Relationship, Drug; Electrophoresis, Polyacrylamide Gel; Glycyrrhetinic Acid - analogs & derivatives; Glycyrrhetinic Acid - pharmacology; glycyrrhetinic acid derivative; glycyrrhizin; histone phosphorylation; Histones - isolation & purification; Histones - metabolism; Phosphorylation - drug effects; Protein Binding; Protein Kinases - pharmacology; Thymus Gland - chemistry; Tumor Cells, Cultured
• ISSN: 0040-8727; 1349-3329
• DOI: 10.1620/tjem.172.123

SHAMSA, F., IWASA, T., SAITO, H., NAGATA, N. and OHTSUKI, K. The Biological Significance of Glycyrrhizin- and Glycyrrhetinic Acid Derivative-Induced Selective Phosphorylation of Histones H2A and H2B by A-kinase In Vitro. Tohoku J. Exp. Med., 1994, 172 (2), 123-132 - Histones H2A and H2B were found to be glycyrrhizin (GL)-binding proteins, because (i) the two histones H2A-H2B pairs were isolated selectively from the crude histone preparations of calf thymus by means of GL-affinity column chromatography (HPLC); (ii) phosphorylation of these two histones by A-kinase was remarkably stimulated by native GL or oGA (a derivative of glycyrrhetinic acid) at 20μM; and (iii) in the crude histone preparations of calf thymus, these two histones were selectively phosphorylated by A-kinase in the presence of both dsDNA and 20μM oGA or 20μM GL. The provided data suggest that the GL-induced selective phosphorylation of histones H2A and H2B by A-kinase may be implicated in the transcriptional activation involved in the biological activities of the drug.