Identification of WU polyomavirus from pediatric patients with acute respiratory infections in Beijing, China

• Published in: Archives of virology Vol. 155; no. 2; pp. 181 - 186
• Main Authors: Zhao, Linqing, Qian, Yuan, Zhu, Runan, Deng, Jie, Wang, Fang, Sun, Yu, Ding, Yaxin
• Format: Journal Article
• Language: English
• Published: Vienna Vienna : Springer Vienna 01.02.2010; Springer Vienna; Springer; Springer Nature B.V
• Subjects: Adolescent; Biological and medical sciences; Biomedical and Life Sciences; Biomedicine; Child; Child, Preschool; China - epidemiology; Cluster Analysis; Comorbidity; DNA, Viral - chemistry; DNA, Viral - genetics; DNA, Viral - isolation & purification; Female; Fluorescent Antibody Technique, Indirect - methods; Fundamental and applied biological sciences. Psychology; Humans; Infant; Infant, Newborn; Infectious Diseases; Male; Medical Microbiology; Microbiology; Miscellaneous; Molecular Sequence Data; Original; Original Article; Phylogeny; Polymerase Chain Reaction - methods; Polyomavirus; Polyomavirus - classification; Polyomavirus - isolation & purification; Polyomavirus Infections - epidemiology; Polyomavirus Infections - virology; Prevalence; Respiratory Tract Infections - epidemiology; Respiratory Tract Infections - virology; Reverse Transcriptase Polymerase Chain Reaction - methods; Sequence Analysis, DNA; Sequence Homology, Amino Acid; Sequence Homology, Nucleic Acid; Viral Structural Proteins - genetics; Virology; Asia; China; Acute Lower Respiratory Tract Infection; Respiratory Syncytial Virus; Throat Swab; Progressive Multifocal Leukoencephalopathy; Acute Respiratory Infection; Virus; Human; Respiratory disease; Acute; Polyomavirus; Identification; Papovaviridae
• ISSN: 0304-8608; 1432-8798
• DOI: 10.1007/s00705-009-0558-7

A novel polyomavirus (WU virus) has been identified in pediatric patients with acute respiratory tract infections (ARI), but its role as a respiratory pathogen has not yet been demonstrated. To investigate if WU virus is related to acute respiratory infections in infants and children in Beijing, specimens collected from 674 pediatric patients with ARI from April 2007 to May 2008 and from 202 children without ARI were used for this investigation. Common respiratory viruses were tested by virus isolation and/or antigen detection by indirect immunofluorescent assay followed by RT-PCR or PCR for other viruses associated with respiratory infections in specimens collected from patients with ARI before WU virus DNA was detected. WU virus DNA was detected by initial screening and secondary confirmation PCR for all specimens. The region encoding the VP2 gene of the virus was amplified from 17 WU-virus-positive clinical specimens, and sequence analysis was performed. Thirty-eight of 674 (5.6%) specimens from patients with ARI and 3 of 202 (1.5%) specimens from children without ARI yielded PCR products with the predicted molecular weight, using either screening or confirmation primer sets, indicating that these specimens were WU virus positive. However, more than 60% of the 38 WU-virus-positive specimens from patients with ARI were also positive for one or more respiratory viruses. The nucleotide and deduced amino acid sequences of the region encoding the VP2 gene from 17 Beijing WU viruses shared high homology (>98.5%) with sequences from GenBank and among themselves. The data indicated that WU virus in Beijing occurred 3.7 times more frequently in pediatric patients with ARI than in those without ARI (p < 0.05).