Multispectral Optoacoustic Tomography (MSOT) of Human Breast Cancer

• Published in: Clinical cancer research Vol. 23; no. 22; pp. 6912 - 6922
• Main Authors: Diot, Gael, Metz, Stephan, Noske, Aurelia, Liapis, Evangelos, Schroeder, Barbara, Ovsepian, Saak V, Meier, Reinhard, Rummeny, Ernst, Ntziachristos, Vasilis
• Format: Journal Article
• Language: English
• Published: United States American Association for Cancer Research Inc 15.11.2017
• Subjects: Adult; Aged; Aged, 80 and over; Biomarkers, Tumor; Blood volume; Breast cancer; Breast Neoplasms - diagnostic imaging; Breast Neoplasms - metabolism; Breast Neoplasms - pathology; Cancer; Case-Control Studies; Deoxygenation; Experimental design; Female; Hemoglobin; High resolution; Humans; Image acquisition; Image contrast; Image Processing, Computer-Assisted; Invasiveness; Magnetic Resonance Imaging; Mammography; Middle Aged; Multimodal Imaging - methods; Neoplasm Grading; Neoplasm Staging; Optics; Photoacoustic Techniques; Precision medicine; Spectra; Tissues; Tomography; Tomography, Optical - methods; Tumors; Ultrasound; Wavelengths
• ISSN: 1078-0432; 1557-3265
• DOI: 10.1158/1078-0432.ccr-16-3200

In a pilot study, we introduce fast handheld multispectral optoacoustic tomography (MSOT) of the breast at 28 wavelengths, aiming to identify high-resolution optoacoustic (photoacoustic) patterns of breast cancer and noncancerous breast tissue. We imaged 10 female patients ages 48-81 years with malignant nonspecific breast cancer or invasive lobular carcinoma. Three healthy volunteers ages 31-36 years were also imaged. Fast-MSOT was based on unique single-frame-per-pulse (SFPP) image acquisition employed to improve the accuracy of spectral differentiation over using a small number of wavelengths. Breast tissue was illuminated at the 700-970 nm spectral range over 0.56 seconds total scan time. MSOT data were guided by ultrasonography and X-ray mammography or MRI. The extended spectral range allowed the computation of oxygenated hemoglobin (HBO ), deoxygenated hemoglobin (HB), total blood volume (TBV), lipid, and water contributions, allowing first insights into high-resolution breast tissue MSOT cancer patterns. TBV and Hb/HBO images resolved marked differences between cancer and control tissue, manifested as a vessel-rich tumor periphery with highly heterogeneous spatial appearance compared with healthy tissue. We observe significant TBV variations between different tumors and between tumors over healthy tissues. Water and fat lipid layers appear disrupted in cancer versus healthy tissue; however, offer weaker contrast compared with TBV images. In contrast to optical methods, MSOT resolves physiologic cancer features with high resolution and revealed patterns not offered by other radiologic modalities. The new features relate to personalized and precision medicine potential. .