Two-stage cultivation of two Chlorella sp. strains by simultaneous treatment of brewery wastewater and maximizing lipid productivity

• Published in: Bioresource technology Vol. 132; pp. 230 - 238
• Main Authors: Farooq, Wasif, Lee, Young-Chul, Ryu, Byung-Gon, Kim, Byung-Hyuk, Kim, Hee-Sik, Choi, Yoon-E., Yang, Ji-Won
• Format: Journal Article
• Language: English
• Published: Kidlington Elsevier Ltd 01.03.2013; Elsevier
• Subjects: Applied sciences; Beer - microbiology; Beers; Biofuel production; Biofuels; Biological and medical sciences; Biomass; Biotechnology; Breweries; brewing industry; Cell Culture Techniques - methods; Chlorella; Chlorella - growth & development; Chlorella - metabolism; Chlorella vulgaris; Contamination control; Cultivation; Energy; Exact sciences and technology; Fermented food industries; Food industries; Fundamental and applied biological sciences. Psychology; Industrial applications and implications. Economical aspects; lipid content; Lipid productivity; Lipids; Lipids - biosynthesis; Microalgal biodiesel; nitrogen content; nutrients; phosphorus; Pollution; Productivity; Species Specificity; Strain; Two-stage cultivation; Waste Disposal, Fluid - methods; Waste water; wastewater; Wastewater treatment; Wastewaters; Water treatment and pollution; Lipid productivity; Contamination control; Microalgal biodiesel; Wastewater treatment; Two-stage cultivation; Brewery; Productivity; Chlorella; Algae; Lipids; Chlorophyceae; Biodiesel; Contamination; Waste water; Chlorophyta; Alga; Microorganism; Waste water purification; Culture
• ISSN: 0960-8524; 1873-2976
• DOI: 10.1016/j.biortech.2013.01.034

[Display omitted] ► Utilization of wastewater before and after anaerobic digestion as nutrient sources. ► A hybrid system to improve algal biofuel economy with wastewater treatment. ► A new two-stage microalgae cultivation mode to increase lipid productivity. ► Better control on bacterial contamination without any pretreatment. A cultivation system in the two-stage photoautotrophic–photoheterotrophic/mixotrophic mode was adapted to maximize lipid productivity of two freshwater strains of Chlorella sp. grown in brewery wastewater (BWW). The endogenous Chlorella sp. isolated from BWW had a higher growth rate than wild-type Chlorella vulgaris (UTEX-265) while C. vulgaris (UTEX-265) had a higher maximal biomass and lipid contents than that of endogenous Chlorella sp., resulting in more than 90% of the inorganic nutrients in both total nitrogen (TN) and phosphorus (TP) was removed during the first stage in the two-stage photoautotrophic–photoheterotrophic mode in each Chlorella sp. The maximal biomass and lipid contents of C. vulgaris (UTEX-265) for single stage photoautotrophic cultivation were 1.5g/L and 18%, respectively. Importantly, during two-stage photoautotrophic–photoheterotrophic cultivation for C. vulgaris (UTEX-265), the biomass was increased to 3.5g/L, and the lipid productivity was increased from 31.1 to 108.0mg/Lday.