Adipose Tissue Secretion and Expression of Adipocyte-Produced and Stromavascular Fraction-Produced Adipokines Vary during Multiple Phases of Weight-Reducing Dietary Intervention in Obese Women

• Published in: The journal of clinical endocrinology and metabolism Vol. 97; no. 7; pp. E1176 - E1181
• Main Authors: Siklova-Vitkova, Michaela, Klimcakova, Eva, Polak, Jan, Kovacova, Zuzana, Tencerova, Michaela, Rossmeislova, Lenka, Bajzova, Magda, Langin, Dominique, Stich, Vladimir
• Format: Journal Article
• Language: English
• Published: United States Endocrine Society 01.07.2012; Copyright by The Endocrine Society
• Subjects: Adipocytes - metabolism; Adipokines - metabolism; Adipokines - secretion; Adipose Tissue - cytology; Adipose Tissue - metabolism; Adipose Tissue - secretion; Adult; Caloric Restriction; Cell Fractionation; Diet, Reducing; Endocrinology and metabolism; Endothelium, Vascular - cytology; Endothelium, Vascular - metabolism; Female; Human health and pathology; Humans; Life Sciences; Middle Aged; Obesity - blood; Obesity - diet therapy; Obesity - metabolism; Stromal Cells - cytology; Stromal Cells - metabolism; Young Adult
• ISSN: 0021-972X; 1945-7197
• DOI: 10.1210/jc.2011-2380

Context: Obesity is associated with altered plasma levels of adipokines involved in the development of insulin resistance and obesity-related metabolic disturbances. Objective: The aim was to investigate diet-induced changes in adipokine production in sc abdominal adipose tissue (SAT) during a 6-month, multiphase, weight-reducing dietary intervention. Design, Setting, Participants, and Interventions: Forty-eight obese women followed a dietary intervention consisting of a very low-calorie diet (VLCD) (1 month), followed by a weight-stabilization (WS) period, which consisted of a low-calorie diet (2 months), and a weight-maintenance diet (3 months). Main Outcome Measures: Before and at the end of the VLCD and WS, samples of plasma and SAT were obtained. In a subgroup of 26 women, secretion of adipokines was determined in SAT explants, and in a subgroup of 22 women, SAT mRNA expression was measured. Results: Body weight decreased and insulin sensitivity increased during the intervention. Plasma levels, SAT mRNA expression, and secretion rates of adipocyte-produced adipokines (leptin, serum amyloid A, and haptoglobin) decreased during the VLCD and increased during the WS period. Adipokines produced mainly from stroma-vascular cells (IL-6, IL-8, IL-10, IL-1Ra, TNFα, plasminogen activator inhibitor-1, and monocyte chemoattractant protein-1) increased or remained unchanged during VLCD and decreased to levels equal to or lower than prediet levels during the WS period. The diet-induced changes in homeostasis model assessment of insulin resistance correlated with changes in leptin plasma levels during VLCD, WS, and the entire dietary intervention period. Conclusions: Diet-induced regulation of adipokine production in SAT differs according to their cellular origin (adipocytes vs. stroma-vascular cells) and diet phase (VLCD vs. WS). Insulin-sensitivity changes were associated only with those of plasma leptin.