Telomeric fingerprinting of the white root rot fungus, Rosellinia necatrix: a useful tool for strain identification

• Published in: FEMS microbiology letters Vol. 217; no. 1; pp. 95 - 101
• Main Authors: Aimi, Tadanori, Kano, Sanae, Yotsutani, Yoshiki, Morinaga, Tsutomu
• Format: Journal Article
• Language: English
• Published: Oxford, UK Blackwell Publishing Ltd 01.11.2002; Blackwell; Oxford University Press
• Subjects: Anastomosis; Ascomycota - classification; Ascomycota - genetics; Ascospores; Biological and medical sciences; Blotting, Southern; Cell death; Chromosomes; Cloning, Molecular; Deoxyribonucleic acid; DNA; DNA Fingerprinting - instrumentation; DNA Fingerprinting - methods; DNA, Fungal - analysis; Double-stranded RNA; Fingerprint; Fingerprinting; Fragments; Fundamental and applied biological sciences. Psychology; Fungal plant pathogens; Fungi; Microbiology; Mycelia; Nuclease; Nucleotide sequence; Phytopathology. Animal pests. Plant and forest protection; Plant Roots - microbiology; Polymerase Chain Reaction; Polymorphism; Polymorphism, Restriction Fragment Length; Restriction fragment length polymorphism; RNA viruses; Root rot; Rosellinia necatrix; Species Specificity; Strains (organisms); Telomere; Telomere - chemistry; Temperature; Time Factors; Variation, races, biotypes, parasitic specialization, genetics; Vegetative incompatibility; Viruses; Fingerprint; Telomere; Anastomosis; Vegetative incompatibility; Plant pathogen; Genetic marker; Fungi; Rosellinia necatrix; Vegetative compatibility; Anastomosis group; Restriction fragment length polymorphism; Fingerprint method; Ascomycetes; Thallophyta
• ISSN: 0378-1097; 1574-6968
• DOI: 10.1111/j.1574-6968.2002.tb11461.x

Abstract The telomere associated DNA sequence pTel46, which was isolated from Coprinus cinereus, was hybridized with Rosellinia necatrix genomic DNA. The DNA fragments hybridized with pTel46 were more sensitive to Bal 31 nuclease. This result suggests that the DNA fragments hybridized with pTel46 were located at the end of chromosomes in R. necatrix. Telomere-linked restriction fragment length polymorphism (RFLP) was found in strains of R. necatrix isolated from various field and single ascospores. Thus, this marker appears to be an excellent tool to show the great polymorphism of R. necatrix. However, RFLP could not be found among several field isolated strains belonging to the same mycelial compatibility group (MCG) isolated in the same field. Therefore the strains belonging to the same MCG might be the same strain that could be anastomosed with each other without cell death except for strain W718 carrying a double-stranded RNA (dsRNA) virus. Therefore the RFLP corresponded to a MCG group, and none of the strains belonging to the same MCG group showed different RFLP in R. necatrix. Moreover, the presence of a kind of dsRNA virus might imply anastomosis between compatible strains.