Prognostic immunohistochemical markers of primary human melanomas

• Published in: British journal of dermatology (1951) Vol. 145; no. 2; pp. 203 - 209
• Main Authors: Ostmeier, H., Fuchs, B., Otto, F., Mawick, R., Lippold, A., Krieg, V., Suter, L.
• Format: Journal Article
• Language: English
• Published: Oxford, UK Blackwell Science Ltd 01.08.2001; Blackwell; Oxford University Press
• Subjects: Adolescent; Adult; Age Factors; Aged; Antigens, CD; Biological and medical sciences; Biomarkers, Tumor - metabolism; CD146 Antigen; Child; Child, Preschool; Dermatology; Disease-Free Survival; Female; Frozen Sections; HLA-DP Antigens - metabolism; HLA-DQ; HLA-DQ Antigens - metabolism; Humans; Infant; Infant, Newborn; Ki-67; Ki-67 Antigen - metabolism; Male; Medical sciences; Melanoma - metabolism; Melanoma - pathology; Membrane Glycoproteins - metabolism; Middle Aged; Mitotic Index; multivariate analysis; Neural Cell Adhesion Molecules; primary malignant melanoma; Prognosis; Receptors, Transferrin - metabolism; Regression Analysis; Sex Factors; Skin Neoplasms - metabolism; Skin Neoplasms - pathology; Tumors of the skin and soft tissue. Premalignant lesions; tumour thickness; Human; Immunohistochemistry; Pathology; HLA-System; Skin disease; Prognosis; Malignant melanoma; Biological marker; Skin; Malignant tumor; Statistical study
• ISSN: 0007-0963; 1365-2133
• DOI: 10.1046/j.1365-2133.2001.04335.x

Background Several clinical and histological factors of primary melanomas comprise a relatively large quantity of prognostic information. Objective To find immunohistochemical markers that can improve the prognostic accuracy achieved by factors that are available without extra laboratory work, i.e. mitotic rate, tumour thickness, ulceration, localization, gender and age. Methods Immunohistochemical markers were determined on frozen sections. Univariate and multivariate Cox regression analyses were performed after 5–10 years follow‐up. Results Seven immunohistochemical markers were related to disease‐free and overall survival in univariate Cox regression analysis: Ki‐67, human leucocyte antigen (HLA) ‐DQ, HLA‐DP, Muc 18, A‐10‐33, transferrin receptor, and H‐2‐8‐10. Only Ki‐67 (n = 399) and HLA‐DQ (n = 452) retained prognostic significance when evaluated in multivariate analyses in several models together with tumour thickness alone and with tumour thickness, gender, mitotic rate, age, localization and ulceration. Conclusions Ki‐67 and HLA‐DQ may be useful for risk assessments in primary melanomas.