Mifepristone modulates serotonin transporter function

Regulating serotonin expression can be used to treat psychotic depression. Mifepristone, a glu- cocorticoid receptor antagonist, is an effective candidate for psychotic depression treatment. However, the underlying mechanism related to serotonin transporter expression is poorly un- derstood. In this...

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Published inNeural regeneration research Vol. 9; no. 6; pp. 646 - 652
Main Authors Li, Chaokun, Shan, Linlin, Jiang, Hongbo, Li, Xinjuan, Wei, Linyu, Li, Dongliang
Format Journal Article
LanguageEnglish
Published India Medknow Publications and Media Pvt. Ltd 15.03.2014
Medknow Publications & Media Pvt. Ltd
Department of Physiology and Neurobiology, Xinxiang Medical University, Xinxiang, Henan Province, China
Medknow Publications & Media Pvt Ltd
Subjects
Antidepressants
Brain research
Cloning
Complications and side effects
Depression, Mental
Dosage and administration
Drug therapy
Electrodes
Genetic aspects
Mental depression
Mifepristone
Psychosis
RNA polymerase
Serotonin
Technical Updates
五羟色胺
受体拮抗剂
抑郁症
皮质激素
米非司酮
调控
转运功能
非洲爪蟾卵母细胞
China
Beijing China
United States > US
two-electrode voltage clamp
current recording
psychotic depression
antidepressants
serotonin transmission
mifepristone
serotonin
neural regeneration
serotonin transporter
nerve regeneration
depression
NSFC grant
Xenopus oocyte
sero-tonin transmission
Online AccessGet full text
ISSN1673-5374
1876-7958
DOI10.4103/1673-5374.130112

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Summary:Regulating serotonin expression can be used to treat psychotic depression. Mifepristone, a glu- cocorticoid receptor antagonist, is an effective candidate for psychotic depression treatment. However, the underlying mechanism related to serotonin transporter expression is poorly un- derstood. In this study, we cloned the human brain serotonin transporter into Xenopus oocytes, to establish an in vitro expression system. Two-electrode voltage clamp recordings were used to detect serotonin transporter activity. Our results show that mifepristone attenuates serotonin transporter activity by directly inhibiting the serotonin transporter, and suggests that the se- rotonin transporter is a pharmacological target of mifepristone for the treatment of psychotic depression.
Bibliography:nerve regeneration; psychotic depression; mifepristone; serownin transporter; Xenopusoocyte; serotonin; depression; two-electrode voltage clamp; current recording; antidepressants; sero-tonin transmission; NSFC grant; neural regeneration
Regulating serotonin expression can be used to treat psychotic depression. Mifepristone, a glu- cocorticoid receptor antagonist, is an effective candidate for psychotic depression treatment. However, the underlying mechanism related to serotonin transporter expression is poorly un- derstood. In this study, we cloned the human brain serotonin transporter into Xenopus oocytes, to establish an in vitro expression system. Two-electrode voltage clamp recordings were used to detect serotonin transporter activity. Our results show that mifepristone attenuates serotonin transporter activity by directly inhibiting the serotonin transporter, and suggests that the se- rotonin transporter is a pharmacological target of mifepristone for the treatment of psychotic depression.
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Author contributions: Li CK designed the study and wrote the manuscript. Shan LL cloned the serotonin transporter. Li XJ and Wei LY performed drug screening using the two-electrode voltage-clamp technique. Li DL designed primers and revised the manuscript. All authors read and approved the final manuscript.
Chaokun Li and Linlin Shan contributed equally to this article.
ISSN:1673-5374
1876-7958
DOI:10.4103/1673-5374.130112