Distribution of membrane progesterone receptor alpha in the male mouse and rat brain and its regulation after traumatic brain injury

• Published in: Neuroscience Vol. 231; pp. 111 - 124
• Main Authors: Meffre, D, Labombarda, F, Delespierre, B, Chastre, A, De Nicola, A.F, Stein, D.G, Schumacher, M, Guennoun, R
• Format: Journal Article
• Language: English
• Published: United States Elsevier Ltd 12.02.2013
• Subjects: Animals; astrocytes; Brain - drug effects; Brain - metabolism; Brain Injuries - genetics; Brain Injuries - metabolism; Cell Membrane - drug effects; Cell Membrane - genetics; Cell Membrane - metabolism; Estradiol - pharmacology; Female; Male; membrane progesterone receptor (mPR); Mice; Mice, Inbred C57BL; microglia; Neuroglia - drug effects; Neuroglia - metabolism; Neurology; neurons; Neurons - drug effects; Neurons - metabolism; oligodendrocytes; Prefrontal Cortex - injuries; Progesterone - pharmacology; Rats; Rats, Sprague-Dawley; Receptors, Progesterone - genetics; Receptors, Progesterone - metabolism; traumatic brain injury (TBI); PR; RT; 3β-HSD; TRIS buffer salin -Tween 20; neurons; antero-posterior; neuronal nuclei; analysis of variance; ANOVA; Inflammatory proteins C3 complement; saline-sodium citrate; sodium dodecyl sulfate; traumatic brain injury (TBI); progesterone receptor membrane component 1; TBI; membrane progesterone receptor (mPR); microglia; diaminobenzidine; membrane progesterone receptor; PBS; SSC; SDS; DAB; Pgrmc1; estrogen receptor; reverse transcriptase; Glial fibrillary acidic protein; traumatic brain injury; TBST; 3β-hydroxysteroid dehydrogenase/Δ5-Δ4-isomerase; progesterone nuclear receptors; oligodendrocytes; CFC3; ER; mPR; AP; GFAP; NeuN; phosphate buffered saline; SEM; standard error of the mean; astrocytes
• ISSN: 0306-4522; 1873-7544
• DOI: 10.1016/j.neuroscience.2012.11.039

Highlights ► mPRα shows a wide distribution in the male rodent brain. ► In basal condition, mPRα is expressed by neurons but not in the glia. ► After TBI, mPRα is induced in astrocytes, oligodendrocytes and activated microglia.