Characterization of the osteoblast-specific transmembrane protein IFITM5 and analysis of IFITM5-deficient mice

• Published in: Journal of bone and mineral metabolism Vol. 29; no. 3; pp. 279 - 290
• Main Authors: Hanagata, Nobutaka, Li, Xianglan, Morita, Hiromi, Takemura, Taro, Li, Jie, Minowa, Takashi
• Format: Journal Article
• Language: English
• Published: Japan Springer Japan 01.05.2011; Springer; Springer Nature B.V
• Subjects: Animals; Animals, Newborn; Biological and medical sciences; Bone and Bones - metabolism; Calcification, Physiologic; Diseases of the osteoarticular system; Embryo, Mammalian - cytology; Embryo, Mammalian - metabolism; Gene Expression Regulation, Developmental; Gene Knockout Techniques; Heterozygote; Homozygote; Medical sciences; Medicine; Medicine & Public Health; Membrane Proteins - deficiency; Membrane Proteins - genetics; Membrane Proteins - metabolism; Metabolic Diseases; Mice; Organ Size; Organ Specificity; Original Article; Orthopedics; Osteoblasts - cytology; Osteoblasts - metabolism; Phenotype; Protein Binding; RNA Transport; RNA, Messenger - genetics; RNA, Messenger - metabolism; Tacrolimus Binding Proteins - metabolism; Knockout mice; Bone growth; Protein interaction; Osteoblasts; IFITM5; Transmembrane protein; Growth; Rodentia; Rheumatology; Vertebrata; Mammalia; Mouse; Animal; Osteoblast; Bone
• ISSN: 0914-8779; 1435-5604
• DOI: 10.1007/s00774-010-0221-0

Interferon-inducible transmembrane protein 5 (IFITM5) is an osteoblast-specific membrane protein whose expression peaks around the early mineralization stage during the osteoblast maturation process. To investigate IFITM5 function, we first sought to identify which proteins interact with IFITM5. Liquid chromatography mass spectrometry revealed that FK506-binding protein 11 (FKBP11) co-immunoprecipitated with IFITM5. FKBP11 is the only protein it was found to interact with in osteoblasts, while IFITM5 interacts with several proteins in fibroblasts. FKBPs are involved in protein folding and immunosuppressant binding, but we could not be sure that IFITM5 participated in these activities when bound to FKBP11. Thus, we generated Ifitm5 -deficient mice and analyzed their skeletal phenotypes. The skeletons, especially the long bones, of homozygous mutants ( Ifitm5 −/− ) were smaller than those of heterozygous mutants ( Ifitm5 +/− ), although we did not observe any significant differences in bone morphometric parameters. The effect of Ifitm5 deficiency on bone formation was more significant in newborns than in young and adult mice, suggesting that Ifitm5 deficiency might have a greater effect on prenatal bone development. Overall, the effect of Ifitm5 deficiency on bone formation was less than we expected. We hypothesize that this may have resulted from a compensatory mechanism in Ifitm5 -deficient mice.