Survival of Mycobacterium avium subsp. paratuberculosis in Dam Water and Sediment

• Published in: Applied and Environmental Microbiology Vol. 71; no. 9; pp. 5304 - 5308
• Main Authors: Whittington, Richard J, Marsh, Ian B, Reddacliff, Leslie A
• Format: Journal Article
• Language: English
• Published: Washington, DC American Society for Microbiology 01.09.2005
• Subjects: agricultural soils; Animals; Australia; Bacteria; Bacteriology; Biological and medical sciences; cattle; cattle manure; Culture Media; Dams; disease transmission; Feces; Feces - microbiology; Fresh Water - chemistry; Fresh Water - microbiology; Fundamental and applied biological sciences. Psychology; Geologic Sediments - microbiology; Groundwater; lacustrine sediments; long term survival; Microbiology; Mycobacterium avium subsp. paratuberculosis; Mycobacterium avium subsp. paratuberculosis - growth & development; Mycobacterium avium subsp. paratuberculosis - isolation & purification; pathogen survival; Pathogenicity, virulence, toxins, bacteriocins, pyrogens, host-bacteria relations, miscellaneous strains; Public Health Microbiology; reservoirs; risk assessment; Seasons; sediment contamination; Sediments; Soil Microbiology; surface water; Temperature; water pollution; waterborne diseases; Weather; New South Wales; Australia; Water; Mycobacteriales; Mycobacteriaceae; Mycobacterium avium; Bacteria; Actinomycetes; Sediments; Survival
• ISSN: 0099-2240; 1098-5336
• DOI: 10.1128/AEM.71.9.5304-5308.2005

In a previous longitudinal study, Mycobacterium avium subsp. paratuberculosis survived for 55 weeks in fecal material in the shade, but for much shorter periods in exposed locations. In this experiment, the survival of the organism was studied in 250 liters of dam water and sediment in large water troughs that were placed in either a semiexposed location or in a shaded location and compared to survival in fecal material and soil in the shaded location. Survival in water and/or sediment in the shade was for up to 48 weeks compared to 36 weeks in the semiexposed location. Survival in sediment was 12 to 26 weeks longer than survival in the water column. Survival in soil and fecal material in the terrestrial environment in the shaded location was only 12 weeks. Although disturbance to sediment could not be ruled out as a factor, there was evidence of dormancy in both the water column and the sediment, since the organism could not be recovered for several months before again becoming detectable. The results suggest that water may be a significant reservoir of M. avium subsp. paratuberculosis infection. Further research on the biology of the organism in aquatic environments is warranted. Animal health authorities will need to provide appropriate advice to farmers to minimize exposure of livestock to potentially infected water sources. Survival of the organism in water destined for human consumption will need to be addressed if the organism is found to be involved in the etiology of Crohn's disease.