Tang-Ping-San Decoction Remodel Intestinal Flora and Barrier to Ameliorate Type 2 Diabetes Mellitus in Rodent Model

• Published in: Diabetes, metabolic syndrome and obesity Vol. 15; pp. 2563 - 2581
• Main Authors: Yin, Wen, Zhang, Si-Qi, Pang, Wen-Lin, Chen, Xiao-Jiao, Wen, Jing, Hou, Jiong, Wang, Cui, Song, Li-Yun, Qiu, Zhen-Ming, Liang, Peng-Tao, Yuan, Jia-Li, Yang, Zhong-Shan, Bian, Yao
• Format: Journal Article
• Language: English
• Published: Macclesfield Dove Medical Press Limited 01.01.2022; Taylor & Francis Ltd; Dove; Dove Medical Press
• Subjects: Analysis; Bacteria; Biotechnology; Chinese medicine; Cholesterol; Diabetes; Diet; Drug dosages; Enzymes; Genetic aspects; Glucose; glycolipid metabolism; gut microbiota; Health aspects; Hyperglycemia; Immunoassay; Inflammation; Insulin resistance; Laboratory animals; Liver; Metabolic disorders; Metabolites; Microbiota; Microbiota (Symbiotic organisms); Morphology; Oral administration; Original Research; Physiological aspects; Physiology; Plants; R&D; Research & development; Streptozocin; tang-ping-san decoction; Tumor necrosis factor-TNF; Type 2 diabetes; type 2 diabetes mellitus; China; United States > US
• ISSN: 1178-7007; 1178-7007
• DOI: 10.2147/DMSO.S375572

Purpose: Type 2 diabetes mellitus (T2DM) is a complex genetic disease associated with genetic and environmental factors. Previous studies have shown that changes in the gut microbiota may affect the development of host metabolic diseases and promote the progression of T2DM. Tang-ping-san (TPS) decoction can effectively treat T2DM. However, its specific mechanisms must be evaluated. Patients and Methods: In the present study, we established an animal model of T2DM using a high-fat diet (HFD) with intraperitoneal injection streptozotocin injection. Results: The therapeutic effect of TPS decoction on T2DM in mice was initially evaluated. TPS decoction was found to improve hyperglycemia, hyperlipidemia, insulin resistance, and pathological liver, pancreatic, and colon changes. Moreover, it reduced the proinflammatory cytokine levels. Based on 16SrRNA sequencing, TPS decoction reduced the Firmicutes/Bacteroidetes ratio at the phylum level. At the genus level, it increased the relative abundances of Akkermansia, Muribaculaceae, and the Eubacterium coprostanoligenes group and decreased the relative abundance of Fusobacterium, Escherichia coli, Dubosiella, and Helicobacter. Conclusion: TPS decoction improves T2DM and liver function and reduces the risk of hyperglycemia, hyperlipidemia, insulin resistance, pathological organ changes, and inflammatory reactions. The mechanism of TPS decoction in T2DM can be correlated with the reversal of gut microbiota dysfunction and repair of the intestinal mucosal barrier. Keywords: type 2 diabetes mellitus, tang-ping-san decoction, gut microbiota, glycolipid metabolism, insulin resistance