Beneficial effects of enteral fat administration on liver dysfunction, liver lipid accumulation, and protein metabolism in septic rats

• Published in: Journal of Nutritional Science and Vitaminology Vol. 41; no. 6; pp. 657 - 669
• Main Authors: Hayashi, N. (Snow Brand Milk Products Co. Ltd., Tochigi (Japan). Life Science Research Inst.), Kashiwabara, N, Yoshihara, D, Takeshita, Y, Handa, H, Yanai, M, Maeda, J, Yamakawa, M
• Format: Journal Article
• Language: English
• Published: Tokyo Center for Academic Publications Japan 1995
• Subjects: Alanine Transaminase - blood; Alkaline Phosphatase - blood; Anesthesia. Intensive care medicine. Transfusions. Cell therapy and gene therapy; Animals; Aspartate Aminotransferases - blood; Biological and medical sciences; Cecum - surgery; CORPS GRAS; Dietary Fats - therapeutic use; Emergency and intensive care: infection, septic shock; ENFERMEDADES HEPATICAS; enteral fat administration; Enteral Nutrition; GRASAS; Intensive care medicine; Ligation; Lipid Metabolism; Lipids - blood; Liver - physiopathology; liver dysfunction; liver lipid accumulation; liver protein; MALADIE DU FOIE; Male; Medical sciences; METABOLISME DES LIPIDES; METABOLISME DES PROTEINES; METABOLISMO DE LIPIDOS; METABOLISMO PROTEICO; Nutritional Status; protein metabolism; Proteins - metabolism; RAT; RATA; Rats; Rats, Wistar; Sepsis - etiology; Sepsis - therapy; septic rats; SEPTICEMIA; SEPTICEMIE; Triglycerides - administration & dosage; Triglycerides - metabolism; Nutrition; Rat; Liver; Rodentia; Lipids; Medium chain; Metabolism; Triglyceride; Enteral administration; Feeding; Infection; Proteins; Liver function; Vertebrata; Experimental disease; Mammalia; Animal; Biological accumulation
• ISSN: 0301-4800; 1881-7742
• DOI: 10.3177/jnsv.41.657

The purpose of this study was to examine the effect of different amounts of fat in enteral diets on liver function, liver lipid accumulation, and protein metabolism in septic rats. Sepsis was induced in Wistar rats by cecal legation and puncture. The rats were divided into four groups and were fed enterally 0% (F0, n=7), 10% (F10, n=7), 20% (F20, n=8), or 30% (F30, n=9) of total calories as fat. The liquid diet consisted of medium-chain and long-chain triglyceride mixtures as the fat sources, casein oligopeptide, and dextrin (100 kcal/100 ml). Intraduodenum feeding was ended on the 6th day. Serum glutamic oxaloacetic transaminase and glutamic pyruvic transaminase activities, indices of liver dysfunction, were highest in the F0 group, and triglycerides accumulated in the livers of that group, possibly because of the large proportion of carbohydrate in the diet. Value of nitrogen balance was highest in the F10 group, and serum total protein and albumin concentration were higher in the F10 and F20 groups than in the F0 and F30 groups. The liver protein content in the F10 and F20 groups was higher than in the F0 and F30 groups. Serum triglyceride in the F30 group was about 2 times higher than in the F10 and F20 groups. These results indicate that enteral fat administration in septic rats as 30% of total calories reduced liver dysfunction and liver triglyceride accumulation, but might have been excessive for optimal protein metabolism. Therefore, the preferable amount may range from 10% to 20% of total calories