A Three-Dimensional Model for the Hammerhead Ribozyme Based on Fluorescence Measurements

• Published in: Science (American Association for the Advancement of Science) Vol. 266; no. 5186; pp. 785 - 789
• Main Authors: Tuschl, Thomas, Gohlke, Christoph, Jovin, Thomas M., Westhof, Eric, Eckstein, Fritz
• Format: Journal Article
• Language: English
• Published: Washington, DC American Society for the Advancement of Science 04.11.1994; American Association for the Advancement of Science
• Subjects: Absorption spectra; Analytical, structural and metabolic biochemistry; Anisotropy; Atoms; Base Composition; Base Sequence; Biological and medical sciences; Catalytic RNA; Dyes; Energy Transfer; Fluoresceins; Fluorescence; Fluorescence spectroscopy; Fundamental and applied biological sciences. Psychology; Least-Squares Analysis; Mathematical helices; Models, Molecular; Molecular biophysics; Molecular Sequence Data; Molecules; Nucleic Acid Conformation; Nucleic acids; Nucleotides; Oligoribonucleotides; Rhodamines; RNA; RNA, Catalytic - chemistry; Rna, ribonucleoproteins; Software; Structure in molecular biology; Tridimensional structure; Three dimensional model; Ribozyme; Double helical structure; Solution structure; Resonant energy transfer; Spatial orientation; Hammerhead structure; Modeling; Structural analysis
• ISSN: 0036-8075; 1095-9203
• DOI: 10.1126/science.7973630

For the understanding of the catalytic function of the RNA hammerhead ribozyme, a three-dimensional model is essential but neither a crystal nor a solution structure has been available. Fluorescence resonance energy transfer (FRET) was used to study the structure of the ribozyme in solution in order to establish the relative spatial orientation of the three constituent Watson-Crick base-paired helical segments. Synthetic constructs were labeled with the fluorescence donor (5-carboxyfluorescein) and acceptor (5-carboxytetramethylrhodamine) located at the ends of the strands constituting the ribozyme molecule. The acceptor helix in helix pairs I and III and in II and III was varied in length from 5 to 11 and 5 to 9 base pairs, respectively, and the FRET efficiencies were determined and correlated with a reference set of labeled RNA duplexes. The FRET efficiencies were predicted on the basis of vector algebra analysis, as a function of the relative helical orientations in the ribozyme constructs, and compared with experimental values. The data were consistent with a Y-shaped arrangement of the ribozyme with helices I and II in close proximity and helix III pointing away. These orientational constraints were used for molecular modeling of a three-dimensional structure of the complete ribozyme.