Pivotal role of protein tyrosine phosphatase 1B (PTP1B) in the macrophage response to pro-inflammatory and anti-inflammatory challenge

• Published in: Cell death & disease Vol. 5; no. 3; p. e1125
• Main Authors: Través, P G, Pardo, V, Pimentel-Santillana, M, González-Rodríguez, Á, Mojena, M, Rico, D, Montenegro, Y, Calés, C, Martín-Sanz, P, Valverde, A M, Boscá, L
• Format: Journal Article
• Language: English
• Published: London Nature Publishing Group UK 13.03.2014; Springer Nature B.V; Nature Publishing Group
• Subjects: 631/250/2504/342; 631/250/516; 631/80/82; 692/699/2743/137; Animals; Antibodies; Biochemistry; Biomedical and Life Sciences; Cell Biology; Cell Culture; Cell Survival; Cells, Cultured; Disease Models, Animal; Galactosamine; Gene Expression Profiling - methods; Humans; Immunity, Innate; Immunology; Inflammation - chemically induced; Inflammation - enzymology; Inflammation - genetics; Inflammation - immunology; Inflammation - pathology; Inflammation Mediators - metabolism; Life Sciences; Lipopolysaccharides; Macrophage Activation; Macrophages, Peritoneal - enzymology; Macrophages, Peritoneal - immunology; Macrophages, Peritoneal - pathology; Male; Mice; Mice, 129 Strain; Mice, Inbred C57BL; Mice, Knockout; Oligonucleotide Array Sequence Analysis; Original; original-article; Protein Tyrosine Phosphatase, Non-Receptor Type 1 - deficiency; Protein Tyrosine Phosphatase, Non-Receptor Type 1 - genetics; Protein Tyrosine Phosphatase, Non-Receptor Type 1 - metabolism; RNA Interference; Signal Transduction; Time Factors; Transfection; Tumor Suppressor Protein p53 - genetics; Tumor Suppressor Protein p53 - metabolism; cell viability; protein tyrosine phosphatase; radiation sensitivity; p53
• ISSN: 2041-4889; 2041-4889
• DOI: 10.1038/cddis.2014.90

Inhibition of protein tyrosine phosphatase 1B (PTP1B) has been suggested as an attractive target to improve insulin sensitivity in different cell types. In the present work, we have investigated the effect of PTP1B deficiency on the response of human and murine macrophages. Using in vitro and in vivo approaches in mice and silencing PTP1B in human macrophages with specific siRNAs, we have demonstrated that PTP1B deficiency increases the effects of pro-inflammatory stimuli in both human and rodent macrophages at the time that decreases the response to alternative stimulation. Moreover, the absence of PTP1B induces a loss of viability in resting macrophages and mainly after activation through the classic pathway. Analysis of early gene expression in macrophages treated with pro-inflammatory stimuli confirmed this exacerbated inflammatory response in PTP1B-deficient macrophages. Microarray analysis in samples from wild-type and PTP1B-deficient macrophages obtained after 24 h of pro-inflammatory stimulation showed an activation of the p53 pathway, including the excision base repair pathway and the insulin signaling pathway in the absence of PTP1B. In animal models of lipopolysaccharide (LPS) and D -galactosamine challenge as a way to reveal in vivo inflammatory responses, animals lacking PTP1B exhibited a higher rate of death. Moreover, these animals showed an enhanced response to irradiation, in agreement with the data obtained in the microarray analysis. In summary, these results indicate that, although inhibition of PTP1B has potential benefits for the treatment of diabetes, it accentuates pro-inflammatory responses compromising at least macrophage viability.