Rapamycin promotes Schwann cell migration and nerve growth factor secretion

Rapamycin, similar to FKS06, can promote neural regeneration in vitro. We assumed that the mechanisms of action of rapamycin and FK506 in promoting peripheral nerve regeneration were similar. This study compared the effects of different concentrations of raparnycin and FK506 on Sc hwann cells and in...

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Published inNeural regeneration research Vol. 9; no. 6; pp. 602 - 609
Main Authors Liu, Fang, Zhang, Haiwei, Zhang, Kaiming, Wang, Xinyu, Li, Shipu, Yin, Yixia
Format Journal Article
LanguageEnglish
Published India Medknow Publications and Media Pvt. Ltd 15.03.2014
Medknow Publications & Media Pvt. Ltd
Department of 0rthopedics, Second Hospital of Yueyang, Yueyang, Hunan Province, China%State Key Laboratory of Advanced Technology for Materials Synthesis and Processing, and Biomedical Materials and Engineering Center, Wuhan University of Technology, Wuhan, Hunan Province, China
Medknow Publications & Media Pvt Ltd
Subjects
Autophagy
Cell adhesion & migration
Cell cycle
Cell growth
Clinical trials
Complications and side effects
Dosage and administration
Drug dosages
FK506
Genetic aspects
Growth factors
Hypertension
Kinases
Ligands
Nerve growth factor
Nervous system
Phosphorylation
Physiological aspects
Proteins
Rapamycin
Regeneration
Research and Report
Schwann cells
分泌
周围神经再生
生长相关蛋白
神经生长因子
细胞迁移
雪旺氏细胞
雷帕霉素
China
neural regeneration
Schwann cell
nerve growth factor
cell migration
growth-associated protein 43
nerve regeneration
peripheral nerve injury
FK506
NSFC grant
rapamycin
cell mi-gration
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Summary:Rapamycin, similar to FKS06, can promote neural regeneration in vitro. We assumed that the mechanisms of action of rapamycin and FK506 in promoting peripheral nerve regeneration were similar. This study compared the effects of different concentrations of raparnycin and FK506 on Sc hwann cells and investigated effects and mechanisms of rapamycin on improving peripheral nerve regeneration. Results demonstrated that the lowest rapamycin concentration （1.53 nmol/L） more significantly promoted Schwann cell migration than the highest FK506 concentration （100μmol/L）. Rapamycin promoted the secretion of nerve growth factors and upregulated growth-associated protein 43 expression in Schwann cells, but did not significantly affect Schwann cell proliferation. Therefore, rapamycin has potential application in peripheral nerve regeneration therapy.
Bibliography:nerve regeneration; peripheral nerve injury; rapamycin; FK506; Schwann cell; cell mi-gration; nerve growth factor; growth-associated protein 43; NSFC grant; neural regeneration
Rapamycin, similar to FKS06, can promote neural regeneration in vitro. We assumed that the mechanisms of action of rapamycin and FK506 in promoting peripheral nerve regeneration were similar. This study compared the effects of different concentrations of raparnycin and FK506 on Sc hwann cells and investigated effects and mechanisms of rapamycin on improving peripheral nerve regeneration. Results demonstrated that the lowest rapamycin concentration （1.53 nmol/L） more significantly promoted Schwann cell migration than the highest FK506 concentration （100μmol/L）. Rapamycin promoted the secretion of nerve growth factors and upregulated growth-associated protein 43 expression in Schwann cells, but did not significantly affect Schwann cell proliferation. Therefore, rapamycin has potential application in peripheral nerve regeneration therapy.
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Author contributions: Liu F and Li SP designed the study. Zhang HW and Yin YX performed experiments. Yin YX wrote the manuscript. All authors participated in data collection and were responsible for quantitative real-time PCR experiments. Zhang KM was responsible for western blot experiments and data analysis. All authors approved the final version of the paper.
ISSN:1673-5374
1876-7958
DOI:10.4103/1673-5374.130101