Transcriptome Signatures of Canine Mammary Gland Tumors and Its Comparison to Human Breast Cancers

Breast cancer (BC)/mammary gland carcinoma (MGC) is the most frequently diagnosed and leading cause of cancer-related mortality in both women and canines. To better understand both canine MGC and human BC-specific genes, we sequenced RNAs obtained from eight pairs of carcinomas and adjacent normal t...

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Published inCancers Vol. 10; no. 9; p. 317
Main Authors Lee, Kang-Hoon, Park, Hyoung-Min, Son, Keun-Hong, Shin, Tae-Jin, Cho, Je-Yoel
Format Journal Article
LanguageEnglish
Published Switzerland MDPI AG 07.09.2018
MDPI
Subjects
Biomarkers
Breast cancer
Carcinoma
Cell fate
Cell proliferation
Comparative analysis
dog
Dogs
Epigenetics
ErbB-2 protein
Gene expression
Gene regulation
Genomes
Growth factors
Homeostasis
Inflammation
Mammary gland
mammary gland tumor
Metastasis
Principal components analysis
RNA-seq
Studies
Transcription
transcriptome
Tumors
Veterinary medicine
mammary gland tumor
breast cancer
RNA-seq
dog
transcriptome
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Summary:Breast cancer (BC)/mammary gland carcinoma (MGC) is the most frequently diagnosed and leading cause of cancer-related mortality in both women and canines. To better understand both canine MGC and human BC-specific genes, we sequenced RNAs obtained from eight pairs of carcinomas and adjacent normal tissues in dogs. By comprehensive transcriptome analysis, 351 differentially expressed genes (DEGs) were identified in overall canine MGCs. Based on the DEGs, comparative analysis revealed correlation existing among the three histological subtypes of canine MGC (ductal, simple, and complex) and four molecular subtypes of human BC (HER2+, ER+, ER&HER2+, and TNBC). Eight DEGs shared by all three subtypes of canine MGCs had been previously reported as cancer-associated genes in human studies. Gene ontology and pathway analyses using the identified DEGs revealed that the biological processes of cell proliferation, adhesion, and inflammatory responses are enriched in up-regulated MGC DEGs. In contrast, fatty acid homeostasis and transcription regulation involved in cell fate commitment were down-regulated in MGC DEGs. Moreover, correlations are demonstrated between upstream promoter transcripts and DEGs. Canine MGC- and subtype-enriched gene expression allows us to better understand both human BC and canine MGC, yielding new insight into the development of biomarkers and targets for both diseases.
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These authors contributed equally to this work.
ISSN:2072-6694
2072-6694
DOI:10.3390/cancers10090317