Subtype-Specific Surface Proteins on Adipose Tissue Macrophages and Their Association to Obesity-Induced Insulin Resistance

A chronic low-grade inflammation, originating in the adipose tissue, is considered a driver of obesity-associated insulin resistance. Macrophage composition in white adipose tissue is believed to contribute to the pathogenesis of metabolic diseases, but a detailed characterization of pro- and anti-i...

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Published inFrontiers in endocrinology (Lausanne) Vol. 13; p. 856530
Main Authors Strand, Kristina, Stiglund, Natalie, Haugstøyl, Martha Eimstad, Kamyab, Zahra, Langhelle, Victoria, Dyer, Laurence, Busch, Christian, Cornillet, Martin, Hjellestad, Iren Drange, Nielsen, Hans Jørgen, Njølstad, Pål Rasmus, Mellgren, Gunnar, Björkström, Niklas K, Fernø, Johan
Format Journal Article
LanguageEnglish
Published Switzerland Frontiers Media S.A 11.04.2022
Subjects
Adipose Tissue - metabolism
adipose tissue macrophage
Endocrinology
flow cytometry
Humans
Inflammation - metabolism
Insulin Resistance
Macrophages - metabolism
Medicin och hälsovetenskap
Membrane Proteins - metabolism
obesity
Obesity - complications
surface proteomics
adipose tissue macrophage
flow cytometry
insulin resistance
surface proteomics
obesity
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Summary:A chronic low-grade inflammation, originating in the adipose tissue, is considered a driver of obesity-associated insulin resistance. Macrophage composition in white adipose tissue is believed to contribute to the pathogenesis of metabolic diseases, but a detailed characterization of pro- and anti-inflammatory adipose tissue macrophages (ATMs) in human obesity and how they are distributed in visceral- and subcutaneous adipose depots is lacking. In this study, we performed a surface proteome screening of pro- and anti-inflammatory ATMs in both subcutaneous- (SAT) and visceral adipose tissue (VAT) and evaluated their relationship with systemic insulin resistance. From the proteomics screen we found novel surface proteins specific to M1-like- and M2-like macrophages, and we identified depot-specific immunophenotypes in SAT and VAT. Furthermore, we found that insulin resistance, assessed by HOMA-IR, was positively associated with a relative increase in pro-inflammatory M1-like macrophages in both SAT and VAT.
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Lead Contact
Edited by: Katherine Samaras, St Vincent’s Hospital Sydney, Australia
Reviewed by: Alexander Bartelt, Ludwig Maximilian University of Munich, Germany; Isabel Casimiro, The University of Chicago, United States
This article was submitted to Obesity, a section of the journal Frontiers in Endocrinology
ISSN:1664-2392
1664-2392
DOI:10.3389/fendo.2022.856530