Human extracellular superoxide dismutase (EC-SOD) expression in transgenic chicken

• Published in: BMB reports Vol. 46; no. 8; pp. 404 - 409
• Main Authors: Byun, S.J., RDA, Suwon, Republic of Korea, Ji, M.R., RDA, Suwon, Republic of Korea, Jang, Y.J., RDA, Suwon, Republic of Korea, Hwang, A.I., RDA, Suwon, Republic of Korea, Chung, H.K., RDA, Suwon, Republic of Korea, Kim, J.S., RDA, Suwon, Republic of Korea, Kim, K.W., RDA, Suwon, Republic of Korea, Chung, H.J., RDA, Suwon, Republic of Korea, Yang, B.C., RDA, Suwon, Republic of Korea, Jeon, I.S., RDA, Suwon, Republic of Korea, Park, J.K., RDA, Suwon, Republic of Korea, Yoo, J.G., RDA, Suwon, Republic of Korea, Kim, T.Y., The Catholic University of Korea, Seoul , Republic of Korea
• Format: Journal Article
• Language: English
• Published: Korea (South) Korean Society for Biochemistry and Molecular Biology 01.08.2013; 생화학분자생물학회
• Subjects: Animals; Animals, Genetically Modified; CHICKENS; Chickens - metabolism; Egg Yolk - metabolism; Extracellular superoxide dismutase; Extracellular superoxide dismutase,Lentiviral vectors; Gene Expression; Gene Transfer Techniques; Genetic Vectors - genetics; Genetic Vectors - metabolism; Humans; Lentiviral vectors; Lentivirus - genetics; ORGANISME TRANSGENIQUE; POLLO; POULET; Recombinant Proteins - biosynthesis; Recombinant Proteins - genetics; Superoxide Dismutase - genetics; Superoxide Dismutase - metabolism; Transgenic; TRANSGENICOS; TRANSGENICS; 화학
• ISSN: 1976-6696; 1976-670X
• DOI: 10.5483/BMBRep.2013.46.8.251

Extracellular superoxide dismutase (EC-SOD) is a metalloprotein and functions as an antioxidant enzyme. In this study, we used lentiviral vectors to generate transgenic chickens that express the human EC-SOD gene. The recombinant lentiviruses were injected into the subgerminal cavity of freshly laid eggs. Subsequently, the embryos were incubated to hatch using phases II and III of the surrogate shell ex vivo culture system. Of 158 injected embryos, 16 chicks (G0) hatched and were screened for the hEC-SOD by PCR. Only 1 chick was identified as a transgenic bird containing the transgene in its germline. This founder (G0) bird was mated with wild-type hens to produce transgenic progeny, and 2 transgenic chicks (G1) were produced. In the generated transgenic hens (G2), the hEC-SOD protein was expressed in the egg white and showed antioxidant activity. These results highlight the potential of the chicken for production of biologically active proteins in egg white.