Upregulated expression and activation of membrane-associated proteases in esophageal squamous cell carcinoma

• Published in: Oncology reports Vol. 31; no. 6; pp. 2820 - 2826
• Main Authors: AUGOFF, KATARZYNA, HRYNIEWICZ-JANKOWSKA, ANITA, TABOLA, RENATA, CZAPLA, LESZEK, SZELACHOWSKI, PIOTR, WIERZBICKI, JAROSLAW, GRABOWSKI, KRZYSZTOF, SIKORSKI, ALEKSANDER F
• Format: Journal Article
• Language: English
• Published: Greece D.A. Spandidos 01.06.2014; Spandidos Publications; Spandidos Publications UK Ltd
• Subjects: Aged; Cancer; Carcinoma, Squamous Cell - genetics; Carcinoma, Squamous Cell - pathology; CD26; Cell adhesion & migration; Development and progression; Dipeptidyl Peptidase 4 - biosynthesis; dipeptidyl peptidase IV; Enzymes; Esophageal cancer; Esophageal Neoplasms - genetics; Esophageal Neoplasms - pathology; Esophageal Squamous Cell Carcinoma; Esophagus; Extracellular matrix; Female; fibroblast activation protein-α; Fibroblasts; Gene Expression Regulation, Neoplastic; Genetic aspects; Growth factors; Humans; Lymphatic system; Male; Matrix Metalloproteinase 14 - biosynthesis; Matrix Metalloproteinase 2 - biosynthesis; Matrix Metalloproteinase 9 - biosynthesis; Medical prognosis; metalloproteinases; Metastasis; Middle Aged; Properties; Proteases; Protein Tyrosine Phosphatase, Non-Receptor Type 13 - biosynthesis; Proteins; seprase; Squamous cell carcinoma; Transcriptional Activation - genetics; Tumors
• ISSN: 1021-335X; 1791-2431
• DOI: 10.3892/or.2014.3162

To better understand the role of membrane- associated proteolytic systems in the development of esophageal cancer, we studied the expression of two serine proteases, fibroblast activation protein-α (FAP-α) and dipeptidyl peptidase IV (DPPIV) and three metalloproteinases, matrix metalloproteinase (MMP)-2, MMP-9 and MT1-MMP in 24 primary esophageal squamous cell carcinoma (ESCC) tissues and paired non-cancer tissues. Using reverse-transcription PCR, western blotting and zymography, we showed that both serine proteases and all three metalloproteinases were highly altered in ESCC. A positive correlation between the expression of FAP-α and DPPIV and the activity of both gelatinases was found. This may indicate that these proteolytic systems are tightly linked to each other and collectively are involved in the process of ECM degradation that facilitates cancer cell invasion and metastasis.