Pancreatic and mucosal enzymes in choline phospholipid digestion

• Published in: American journal of physiology: Gastrointestinal and liver physiology Vol. 316; no. 4; pp. G425 - G445
• Main Authors: Nilsson, Åke, Duan, Rui-Dong
• Format: Journal Article
• Language: English
• Published: United States American Physiological Society 01.04.2019
• Subjects: Acyltransferase; Animals; Bile; Biodegradation; Cell Membrane - physiology; Ceramidase; Ceramide; Cholesterol; Choline; Choline - metabolism; choline phospholipids; clinical implications; Clinical Medicine; Digestion; Digestive system; Digestive System Physiological Phenomena; Energy metabolism; Energy Metabolism - physiology; Enzymes; Gastroenterologi; Gastroenterologi och hepatologi; Gastroenterology and Hepatology; Gastrointestinal Microbiome - physiology; Gastrointestinal tract; Homeostasis; Humans; Inflammation; Intermediates; Intestinal Mucosa - enzymology; Klinisk medicin; Lecithin; Lipase; Lipid metabolism; Lipids; Liver diseases; Medical and Health Sciences; Medicin och hälsovetenskap; Metabolism; Metabolites; Mucosa; mucosal enzymes; Pancreas; Pancreas - enzymology; pancreatic enzymes; Phosphatidic acid; Phosphatidylcholine; Phosphatidylcholines - metabolism; Phospholipase A2; Small intestine; Sphingomyelins - metabolism; trimethylamine; trimethylamine; clinical implications; pancreatic enzymes; choline phospholipids; mucosal enzymes
• ISSN: 0193-1857; 1522-1547; 1522-1547
• DOI: 10.1152/ajpgi.00320.2018

The digestion of choline phospholipids is important for choline homeostasis, lipid signaling, postprandial lipid and energy metabolism, and interaction with intestinal bacteria. The digestion is mediated by the combined action of pancreatic and mucosal enzymes. In the proximal small intestine, hydrolysis of phosphatidylcholine (PC) to 1-lyso-PC and free fatty acid (FFA) by the pancreatic phospholipase A 2 IB coincides with the digestion of the dietary triacylglycerols by lipases, but part of the PC digestion is extended and must be mediated by other enzymes as the jejunoileal brush-border phospholipase B/lipase and mucosal secreted phospholipase A 2 X. Absorbed 1-lyso-PC is partitioned in the mucosal cells between degradation and reacylation into chyle PC. Reutilization of choline for hepatic bile PC synthesis, and the reacylation of 1-lyso-PC into chylomicron PC by the lyso-PC-acyl-CoA-acyltransferase 3 are important features of choline recycling and postprandial lipid metabolism. The role of mucosal enzymes is emphasized by sphingomyelin (SM) being sequentially hydrolyzed by brush-border alkaline sphingomyelinase (alk-SMase) and neutral ceramidase to sphingosine and FFA, which are well absorbed. Ceramide and sphingosine-1-phosphate are generated and are both metabolic intermediates and important lipid messengers. Alk-SMase has anti-inflammatory effects that counteract gut inflammation and tumorigenesis. These may be mediated by multiple mechanisms including generation of sphingolipid metabolites and suppression of autotaxin induction and lyso-phosphatidic acid formation. Here we summarize current knowledge on the roles of pancreatic and mucosal enzymes in PC and SM digestion, and its implications in intestinal and liver diseases, bacterial choline metabolism in the gut, and cholesterol absorption.