Tetrahydropteridines possess antioxidant roles to guard against glucose-induced oxidative stress in Dictyostelium discoideum

• Published in: BMB reports Vol. 46; no. 2; pp. 86 - 91
• Main Authors: Park, S.O., Inje University, Kimhae, Republic of korea, Kim, H.L., Inje University, Kimhae, Republic of korea, Lee, S.W., Inje University, Kimhae, Republic of korea, Park, Y.S., Inje University, Kimhae, Republic of korea
• Format: Journal Article
• Language: English
• Published: Korea (South) Korean Society for Biochemistry and Molecular Biology 01.02.2013; 생화학분자생물학회
• Subjects: Antioxidants - metabolism; DICTYOSTELIUM; Dictyostelium - drug effects; Dictyostelium - growth & development; ESTRES OXIDATIVO; GLUCOSA; GLUCOSE; Glucose - pharmacology; Glycolysis; GTP Cyclohydrolase - metabolism; MITOCHONDRIA; Mitochondria - metabolism; MITOCHONDRIE; MITOCONDRIA; OXIDATIVE STRESS; Oxidative Stress - drug effects; Pteridines - metabolism; Reactive Oxygen Species - metabolism; RNA, Messenger - metabolism; STRESS OXYDATIF; Tetrahydrofolate Dehydrogenase - metabolism; Tetrahydropteridines; 화학
• ISSN: 1976-6696; 1976-670X
• DOI: 10.5483/BMBRep.2013.46.2.128

Glucose effects on the vegetative growth of Dictyostelium discoideum Ax2 were studied by examining oxidative stress and tetrahydropteridine synthesis in cells cultured with different concentrations (0.5X, 7.7 g L-1; 1X, 15.4 g L-1; 2X, 30.8 g L-1) of glucose. The growth rate was optimal in 1X cells (cells grown in 1X glucose) but was impaired drastically in 2X cells, below the level of 0.5X cells. There were glucose-dependent increases in reactive oxygen species (ROS) levels and mitochondrial dysfunction in parallel with the mRNA copy numbers of the enzymes catalyzing tetrahydropteridine synthesis and regeneration. On the other hand, both the specific activities of the enzymes and tetrahydropteridine levels in 2X cells were lower than those in 1X cells, but were higher than those in 0.5X cells. Given the antioxidant function of tetrahydropteridines and both the beneficial and harmful effects of ROS, the results suggest glucose-induced oxidative stress in Dictyostelium, a process that might originate from aerobic glycolysis, as well as a protective role of tetrahydropteridines against this stress.